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Figure 7 | Frontiers in Zoology

Figure 7

From: Cell lineage analysis of the mandibular segment of the amphipod Orchestia cavimana reveals that the crustacean paragnaths are sternal outgrowths and not limbs

Figure 7

In-vivo labeling and clonal composition of area III using the example of cell E(0)p4. (A) Ventral view (double exposure) of the right head region. The lateral part of the mandible is formed by descendants of E(0)p4 as well as adjacent parts of the tergite (white dotted line). Postero-lateral parts of the mandible and parts of the adjacent tergite (Ter) is formed by descendant of cell E(0)p4. (B) SEM-picture of an embryo in an almost the same stage as the embryo shown in A. Only the triangular shaped mandible (Md) and not the anlagen of the paragnaths are developed. The dotted line indicates the slightly deeper medial cells of region E(0). (C) The same embryo as in (A) further developed. Imaris-reconstruction of the left mouth region of the fixed embryo. The blue spots are the counter-stained (Hoechst) nuclei. The E(0)p4-clones form the outer (lateral) tip of the young mandible. The rest of the clones (visible in A) were cut off. For detail see Additional file 3. (antenna 1 = A1; antenna 2 = A2; labrum = lb; maxilla 1 = Mx1; maxilla 2 = Mx2; maxilliped = Mxp; palpus = P; stomodaeum = Sto)

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