Figure 6

In-vivo labeling of area II in region E(0). The images are in ventral view and double exposure. (A-C) Labeling of cell E(0)a2 of the left body half. (A)Anterior embryonic region which at this time lacks visible paragnath buds. The descendants of E(0)a2 form anterior parts of the mandible bud (Md) as well as parts of the developing sternite. (B) Left gnathal region of the embryo as in A further developed. The small palp (P) of the maxilla 1 (Mx1) is now formed. There are two distinct clone domains. One forms anterior part of the small paragnath bud (Pg), the other forms anterior parts of the developing mandible (Md). (C) Left mandibular region of the embryo as in B after further development. The paragnaths grow out and the mandible differentiates into two lobes. The clonal composition of the descendants of E(0)a2 is more or less the same as in (E). (D-E) Labeling of cell E(0)p3 of the left body half. (D) Ventral view of the mouth region. The descendants of the marked cell form posterior parts of the mandibular bud (Md), but they do not form parts of the anlage of the paragnath (Pg). The arrow head shows the oil drop of the marking. (E) Same embryo as in D slightly further developed. Imaris-reconstruction of the left gnathal region. The blue spots represent the counter-stained (Hoechst) nuclei. The descendants of E(0)p3 are involved in the formation of posterior parts of the mandible, which now consists of two well developed lobes. For detail see Additional file 2.