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Figure 2 | Frontiers in Zoology

Figure 2

From: Xenopus embryonic epidermis as a mucociliary cellular ecosystem to assess the effect of sex hormones in a non-reproductive context

Figure 2

Cellular arrangement within the mucociliary epithelium (MCE) of Xenopus embryonic epidermis and ectodermal explants. A,B) Typical MCE arrangement (skin st 30) with a layer of MS cells and interspersed MC and MR cells. Cell populations are marked by fluorescent ISH and/or IHC as follows MC cells (tuba1a-b, ac-Tuba), MR cells (atp6v1a), and MS cells (itln1, Itln1), and imaged by confocal microscopy. C) SEM micrograph of skin st 40 showing the MCE cellular arrangement with MC, MR (rMR and vMR) and a predominant amount of MS cells. D) Abundance of MC, MR and MS cell populations as identified by biomolecular markers in skin at st 30. E) Abundance of MC, rMR, vMR and MS cell populations as identified by SEM in skin at st 40. F-H) Specified (st 15, F) and differentiated (st 30, G,H) MCE in ectodermal explants showing a similar MC, MR and MS cellular arrangement to the MCE in the skin of whole-embryos; p63 (IHC) is a marker of inner-layer basal cells in the skin. A,B, H) 2 μm deep stack; F,G) single optical sections through the explants. H) surface view of the explant. MC, multiciliated; rMR or vMR, ridged or vesicle mitochondrion-rich; MS, mucus-secreting.

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