Figure 7

Morphology hatchlings of Meara stichopi : FMRFamide signal. Different optical sections through a hatchling of Meara stichopi labeled with anti-tyrosinated tubulin (magenta) and anti-FMRFamide (cyan) antibodies, anterior to the left. A) Dorsal section shows neurite bundles (dnb). A basiepidermal ‘commissural’ neurite bundle (cnb) is connecting the two bilateral longitudinal bundles. The longitudinal neurites extend to the posterior end, where the two strands are connected. The dorsal crossing nerves are visible (crn). B) More ventral optical section of the confocal stack. The basiepidermal nerve net (bepnn) is visible and FMRFamide-signal is detected internally around the double statocyst. C) Ventral optical section of the same hatchling as in A) and B). Subepidermal cells that are labeled with the anti-FMRFamide antibody are visible (seamidc). The nature of these cells remains unclear. Scale bar 20 μm.