Litter size manipulation in laboratory mice: an example of how proteomic analysis can uncover new mechanisms underlying the cost of reproduction

Table 4 Separate ANOVAs conducted on PC1 and PC2 values of individual reproductive female mice

	PC1			PC2
	Calcium metabolism and ageing			Cell growth regulation
	F	d.f.	P	F	d.f.	P
Fixed effects
Litter size manipulation	20.68	2, 11	<0.001	4.89	2, 11	0.036
Covariates
Final litter size	0.20	1, 11	0,67	1.81	1, 11	0.22
Final female body mass	0.01	1, 11	0.91	1,15	1, 11	0,32

Final litter size and female body mass at the end of the experiment (n = 12, 4 females in each group) were initially included as covariates (P values indicated in brackets) but were dropped sequentially to obtain the final model. Bold values indicate significant effects.

ISSN: 1742-9994