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Table 2 Results of separate ANOVAs conducted on each protein spot detected.

From: Litter size manipulation in laboratory mice: an example of how proteomic analysis can uncover new mechanisms underlying the cost of reproduction

Spot N° Dependent variables Acc. N° Biological activity F P (ANOVA)
657 Indolethylamine N-methyltransferase gi|731019 Methylation, ageing 7.80 0.011
516 Fructose-1,6-biphosphatase 1 gi|14547989 Carbohydrate metabolism, calcium chelation 7.58 0.012
570 Glycine N-methytransferase gi|15679953 Biogenesis, methylation, carbohydrate metabolism 6.12 0.021
Malate dehydrogenase 1 gi|148675904
422 Alpha-enolase gi|13637776 Cell growth, biogenesis, methylation 5.19 0.032
Adenosylhomocysteinase gi|21431841
553 Dimethylarginine dimethylaminohydrolase 1 gi|45476974 Regulation of nitric oxide generation 4.83 0.038
383 Selenium-binding protein 1 gi|148840436 Cell differentiation 4.51 0.044
748 Regucalcin gi|2498920 Calcium homeostasis, ageing 4.46 0.045
355/365 Catalase gi|157951741 Response to oxidative stress 0.01/0.03 0.99/0.97
437 Ndufs 2 protein gi|13278096 Response to oxidative stress 0.08 0.93
637 Hydroxyacyl glutathione hydrolase gi|13435786 Response to oxidative stress 1.10 0.37
Carbonic anhydrase 3 gi|148673185
663/672 Peroxiredoxin 6 gi|6671549 Response to oxidative stress 0.73/0.77 0.51/0.49
702/791 Peroxiredoxin 1 gi|547923 Response to oxidative stress 0.73/0.38 0.51/0.69
843/850 Glutathione transferase gi|193703 Response to oxidative stress 0.05/0.11 0.95/0.90
681/683 Glutathione transferase zeta 1 gi|148670978 Response to oxidative stress 0.91/0.06 0.44/0.94
702 SOD 2 protein gi|17390379 Response to oxidative stress 0.73 0.51
665/853 Glutathione S transferase Mu 1 gi|121716 Response to oxidative stress 0.17/0.42 0.85/0.67
665/673 Glutathione S transferase Mu 2 gi|121718 Response to oxidative stress 0.17/0.80 0.85/0.48
853 Glutathione S transferase Mu 3 gi|121720 Response to oxidative stress 0.42 0.67
850 Glutathione S transferase Kappa 1 gi|47116757 Response to oxidative stress 0.11 0.90
680 Glutathione S transferase theta-1 gi|160298219 Response to oxidative stress 0.68 0.53
791 Glutathione S-transferase P 1 gi|121747 Response to oxidative stress 0.38 0.69
681 Thioredoxin-dependent peroxide reductase gi|126986 Response to oxidative stress 0.91 0.44
639 Carbonic anhydrase 2 gi|146345383 Response to oxidative stress 0.80 0.48
386/390/393/633/637/647/649/778 Carbonic anhydrase 3 gi|148673185 Response to oxidative stress 0.22/0.07/0.19/0.29/1.10/0.10/0.58/0.36 0.81/0.93/0.83/0.76/0.37/0.90/0.58/0.71
  1. Data were collected in reproductive females belonging to FEL, FRL and Control groups (n = 4 per group). Multiple ANOVAs were used to determine the spots that were likely to be affected by the experimental treatment, and that then entered the subsequent statistical analysis (see Materials and methods for details). Only 7 protein spots containing 9 different proteins were found to be significantly affected by the litter manipulation (in bold). The proteins related to oxidative balance that were detected in the proteomic analysis of the liver samples but which were apparently not affected by litter size are also indicated. Spot N° correspond to the protein spots reported in Additional file 1: Figure S1. The biological activity of proteins as determined using the so-called biological process ontologies (http://www.geneontology.org/) were automatically extracted using the MSDA software suite (https://msda.unistra.fr), and complemented with literature examination.