Litter size manipulation in laboratory mice: an example of how proteomic analysis can uncover new mechanisms underlying the cost of reproduction

Plumel, Marine I; Stier, Antoine; Thiersé, Danièle; van Dorsselaer, Alain; Criscuolo, François; Bertile, Fabrice

doi:10.1186/1742-9994-11-41

Frontiers in Zoology

Table 2 Results of separate ANOVAs conducted on each protein spot detected.

From: Litter size manipulation in laboratory mice: an example of how proteomic analysis can uncover new mechanisms underlying the cost of reproduction

Spot N°	Dependent variables	Acc. N°	Biological activity	F	P (ANOVA)
657	Indolethylamine N-methyltransferase	gi\|731019	Methylation, ageing	7.80	0.011
516	Fructose-1,6-biphosphatase 1	gi\|14547989	Carbohydrate metabolism, calcium chelation	7.58	0.012
570	Glycine N-methytransferase	gi\|15679953	Biogenesis, methylation, carbohydrate metabolism	6.12	0.021
570	Malate dehydrogenase 1	gi\|148675904	Biogenesis, methylation, carbohydrate metabolism	6.12	0.021
422	Alpha-enolase	gi\|13637776	Cell growth, biogenesis, methylation	5.19	0.032
422	Adenosylhomocysteinase	gi\|21431841	Cell growth, biogenesis, methylation	5.19	0.032
553	Dimethylarginine dimethylaminohydrolase 1	gi\|45476974	Regulation of nitric oxide generation	4.83	0.038
383	Selenium-binding protein 1	gi\|148840436	Cell differentiation	4.51	0.044
748	Regucalcin	gi\|2498920	Calcium homeostasis, ageing	4.46	0.045
355/365	Catalase	gi\|157951741	Response to oxidative stress	0.01/0.03	0.99/0.97
437	Ndufs 2 protein	gi\|13278096	Response to oxidative stress	0.08	0.93
637	Hydroxyacyl glutathione hydrolase	gi\|13435786	Response to oxidative stress	1.10	0.37
637	Carbonic anhydrase 3	gi\|148673185	Response to oxidative stress	1.10	0.37
663/672	Peroxiredoxin 6	gi\|6671549	Response to oxidative stress	0.73/0.77	0.51/0.49
702/791	Peroxiredoxin 1	gi\|547923	Response to oxidative stress	0.73/0.38	0.51/0.69
843/850	Glutathione transferase	gi\|193703	Response to oxidative stress	0.05/0.11	0.95/0.90
681/683	Glutathione transferase zeta 1	gi\|148670978	Response to oxidative stress	0.91/0.06	0.44/0.94
702	SOD 2 protein	gi\|17390379	Response to oxidative stress	0.73	0.51
665/853	Glutathione S transferase Mu 1	gi\|121716	Response to oxidative stress	0.17/0.42	0.85/0.67
665/673	Glutathione S transferase Mu 2	gi\|121718	Response to oxidative stress	0.17/0.80	0.85/0.48
853	Glutathione S transferase Mu 3	gi\|121720	Response to oxidative stress	0.42	0.67
850	Glutathione S transferase Kappa 1	gi\|47116757	Response to oxidative stress	0.11	0.90
680	Glutathione S transferase theta-1	gi\|160298219	Response to oxidative stress	0.68	0.53
791	Glutathione S-transferase P 1	gi\|121747	Response to oxidative stress	0.38	0.69
681	Thioredoxin-dependent peroxide reductase	gi\|126986	Response to oxidative stress	0.91	0.44
639	Carbonic anhydrase 2	gi\|146345383	Response to oxidative stress	0.80	0.48
386/390/393/633/637/647/649/778	Carbonic anhydrase 3	gi\|148673185	Response to oxidative stress	0.22/0.07/0.19/0.29/1.10/0.10/0.58/0.36	0.81/0.93/0.83/0.76/0.37/0.90/0.58/0.71

Data were collected in reproductive females belonging to FEL, FRL and Control groups (n = 4 per group). Multiple ANOVAs were used to determine the spots that were likely to be affected by the experimental treatment, and that then entered the subsequent statistical analysis (see Materials and methods for details). Only 7 protein spots containing 9 different proteins were found to be significantly affected by the litter manipulation (in bold). The proteins related to oxidative balance that were detected in the proteomic analysis of the liver samples but which were apparently not affected by litter size are also indicated. Spot N° correspond to the protein spots reported in Additional file 1: Figure S1. The biological activity of proteins as determined using the so-called biological process ontologies (http://www.geneontology.org/) were automatically extracted using the MSDA software suite (https://msda.unistra.fr), and complemented with literature examination.

Back to article page

ISSN: 1742-9994

Contact us

Submission enquiries: Access here and click Contact Us
General enquiries: info@biomedcentral.com