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Table 1 ANOVAs for Global parameters in litters and reproductive adult females

From: Litter size manipulation in laboratory mice: an example of how proteomic analysis can uncover new mechanisms underlying the cost of reproduction

Variable Covariates Repeated factor Random factor Control litters Enlarged litters Reduced litters   P-value
P-value P-value P-value (n = 4) (n = 4) (n = 4)
  [Estimates] [Estimates] [Estimates] [Estimates] [Estimates] [Estimates]   
(a) Litter parameters
Initial litter size (pup number)     8.3 ± 0.8 6.8 ± 0.5 7.3 ± 0.9 F2,11 = 1.61 0.253
[0] [-2.0 ± 1.2] [-0.5 ± 1.2]
Final litter size (pup number)     7.0 ± 0.9 9.3 ± 0.6 4.5 ± 0.7 F2,11 = 5.97 0.022
[0] [1.0 ± 1.1] [-2.8 ± 1.1]
Final Litter mass (g) Final litter size    49.9 ± 4.0 45.4 ± 4.7 59.0 ± 5.3 F2,11 = 1.35 0.313
P = 0.001 [0] [-4.5 ± 5.8] [9.1 ± 7.2]
  [8.3 ± 1.6]        
(b) Adult female parameters
Initial body mass (g) Initial litter mass Stage Individual 27.4 ± 2.6 28.5 ± 2.6 31.7 ± 2.6 F2,8.2 = 4.00 0.061
    26.5 ± 2.6 27.7 ± 2.6 30.8 ± 2.6
Final body mass (g) P = 0.040 P = 0.225   27.0 ± 2.5 28.1 ± 2.6 31.2 ± 2.5
Mean female body mass (g) [0.2 ± 0.1] [3.5 ± 2.8] [4.1 ± 3.6] [0] [1.40 ± 1.8] [4.9 ± 1.8]
Final oxidative stress (mg H2O2/mL) Antioxidant capacity    10.5 ± 0.7 12.4 ± 0.7 10.0 ± 0.7 F2,11 = 5.57 0,030
P = 0.002 [0] [1.9 ± 0.8] [-0.8 ± 0.8]
  [0.1 ± 0.1]        
  1. Results of ANOVAs for global parameters in litters (a) and reproductive adult females (b) subjected to litter size manipulation (Control, FEL and FRL groups). “Initial” refers to pre-reproduction (i.e. 3 weeks before pairing) while “Final” refers to offspring emancipation (Day 21) characteristics. Analysis of variation in adult female body mass over time and treatment was done using a Mixed Model (see Statistic description for details).