Figure 3From: Medaka villin 1-like protein (VILL) is associated with the formation of microvilli induced by decreasing salinities in the absorptive ionocytesThe VILL located to the apical region of the FW-type ionocytes. Confocal micrographs of the whole-mount double immunofluorescence staining with anti-VILL (green; A and B) and anti-NKA antibodies (red) on the afferent sides of gill filaments in the brackish medaka acclimated to FW or SW. The merged images (C and D) revealed that the VILL protein was localized to the NKA-immunoreactive (NKA-IR) cells in the FW fish but not in the SW fish. (E) The magnified merged 3D image of the FW fish gill revealed VILL signals in the apical region of NKA-IR cells. Ex: external environment.Back to article page