Validation of the 3D reconstructed maculae based on microCT imaging. 3D reconstructed macula sacculi (A) and macula lagenae (B) of the herein studied black molly are compared with a corresponding reconstruction from the Atlantic molly Poecilia mexicana in a previous study . Reconstructions of the maculae of P. mexicana (C-E) based on histological serial sections (thickness of sections: 1 μm). The shape of the respective macula types of black molly and Atlantic molly show a high degree of similarity, especially with regard to the macula sacculi, indicating the reliability of 3D reconstructed sensory epithelia based on microCT imaging. Similar features of the maculae sacculi are marked by the arrow and circle. The yellow area in (C) represents reconstructed non-sensory epithelium surrounding the macula sacculi. All maculae are shown in lateral view with their dorsal side up and anterior to the right. Scale bars, 100 μm.