Table 2 The treatment of living gregarines with jasplakinolide
From: The enigma of eugregarine epicytic folds: where gliding motility originates?
Species/JAS concentration | Gregarina cuneata | Gregarina polymorpha | Gregarina steini | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
5 μM JAS | 10 μM JAS | 20 μM JAS | 30 μM JAS | 5 μM JAS | 10 μM JAS | 20 μM JAS | 30 μM JAS | 5 μM JAS | 10 μM JAS | 20 μM JAS | 30 μM JAS | |
Changes/time left after drug application (in minutes) | ||||||||||||
Initial increase of gliding speed | ≥ 5 min+ | ≥ 5 min+ | ≥ 5 min++ | ≥ 5 min++ | ≥ 5 min+ | ≥ 5 min+ | ≥ 5 min++ | ≥ 5 min++ | ≥ 5 min+ | ≥ 5 min+ | ≥ 5 min++ | ≥ 5 min++ |
Decrease of gliding speed to the normal rate | ≥ 90 min+ | ≥ 20 min++ | ≥ 20 min++ | ≥ 20 min++ | ≥ 90 min+ | ≥ 20 min++ | ≥ 20 min++ | ≥ 20 min++ | ≥ 90 min+ | ≥ 20 min++ | ≥ 20 min++ | ≥ 20 min++ |
Further decrease of gliding speed | ≥ 100 min+ | ≥ 30 min++ | ≥ 30 min++ | ≥ 30 min++ | ≥ 100 min+ | ≥ 30 min++ | ≥ 30 min++ | ≥ 30 min++ | ≥ 100 min+ | ≥ 30 min+ | ≥ 30 min++ | ≥ 30 min++ |
Cellular changes (shrivelling) | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min | ≥ 60 min |
Complete stoppage of gliding motility | ≤ 150 min | ≤ 140 min | ≤ 100 min | ≤ 100 min | ≤ 150 min | ≤ 140 min | ≤ 100 min | ≤ 100 min | ≤ 130 min | ≤ 120 min | ≤ 90 min | ≤ 90 min |
Recovery of cell shape after washing in Ringer’s solution | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min | ≤ 1 min |
Full recovery of motility after washing in Ringer’s solution | ≥ 5 min | ≥ 5 min | ≥ 5 min | ≥ 5 min | ≤ 10 min | ≥ 10 min | ≥ 10 min | ≥ 10 min | ≤ 30 min | ≥ 30 min | ≥ 30 min | ≥ 30 min |