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Figure 2 | Frontiers in Zoology

Figure 2

From: The enigma of eugregarine epicytic folds: where gliding motility originates?

Figure 2

Actin and myosin in Gregarina polymorpha gamonts. A. Gamonts in syzygy; primite (p), satellite (s). LM, transmitted light. B. F-actin localisation in a gamont; nucleus (asterisk), septum (arrowhead). CLSM, phalloidin-TRITC. C-D. F-actin localisation in gamonts (previously associated in syzygy) treated for 10 minutes with 10 μM JAS. The more intense labelling is restricted to the cortex; septum (arrowhead), nucleus (asterisk). Figure C shows a primite. CLSM (left) and merged CLSM/transmitted light (right), phalloidin-TRITC. Figure D shows a satellite. CLSM, phalloidin-TRITC. E. The deutomerite of a gamont treated for 10 minutes with 10 μM JAS. F-actin localisation corresponds to the cortex; nucleus (asterisk). Upper two figures show the gamont middle plane; lower figure shows the cortex in the area of epicytic folds. Merged CLSM/transmitted light (upper) and CLSM, phalloidin-TRITC. F. F-actin localisation in a gamont treated for 150 minutes with 10 μM JAS, showing decreased labelling of cortex and septum (arrowhead). Numerous cytoplasmic F-actin aggregations give the labelling homogenous appearance. CLSM, phalloidin-TRITC. G. The deutomerite of a gamont treated for 150 minutes with 10 μM JAS; rosette-like aggregations of F-actin (arrows), nucleus (asterisk). Merged CLSM/transmitted light (upper) and CLSM (lower), phalloidin-TRITC. H. Actin localisation in a gamont; nucleus (asterisk), septum (arrowhead). The indistinct labelling (green) is more evident in the cortex covering the anterior part of cell. CLSM, IFA. I–J. Myosin localisation in a gamont. The labelling is restricted to the cortex, with a pattern of longitudinal rows; septum (arrowhead). CLSM, IFA. K. The anterior part of the gamont shown in J. Myosin localisation is restricted to the cortex, but not to the septum (arrowhead). Merged CLSM/transmitted light, IFA. L. Myosin localisation in a gamont ghost; septum (arrowhead). CLSM, IFA. Figures H, J and L show merged FITC (antibody) and rhodamine (counterstaining with Evans blue) fluorescence channels.

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