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Figure 6 | Frontiers in Zoology

Figure 6

From: Development in a naturally acidified environment: Na+/H+-exchanger 3-based proton secretion leads to CO2 tolerance in cephalopod embryos

Figure 6

Measurement of epidermal proton gradients. Proton gradients were recorded on 6 spots along the lateral side of control and CO2 acclimated embryos (A + B). Ion selective electrode technique measurements demonstrate that under control conditions the entire animal including yolk, head and mantle has positive ∆[H+] values with highest levels detected in the head region (spot 4 and 5) (B). Lower H+ secretion values were recorded for the entire yolk surface. When exposed to elevated seawater p CO2 proton secretion increases significantly on the yolk. Increases in H+ gradients on the yolk, evoked by CO2 stimulation are sensitive to 1 μM EIPA (C). Asterisks indicate significant differences between treatments (p < 0.02). Bars represent mean ± SE; (n = 3; with the average of 1–2 animals from each replicate).

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