Postural components of the Transport Response. The typical posture during manual carrying of the undisturbed (No Anesthesia) pups at PND 10 (A) and PND 14 (B) compared with a PND 14 pup under general anesthesia with a 40 mg/kg pentobarbital injection (C). (D–F) The mean ± SEM of the circularity of the lumber region (D), the length from the nose to the toe (E) and the dorsal body length in contact with a tube normalized to the whole body length (F, G) during the immobilization period in the undisturbed and anesthetized pups at PND 14 are shown. n = 12 per group. (H) The mean ± SEM width between the upper and lower eyelids at 0, 2 and 4 s after the onset of immobility using PND 16 pups (n = 13). *p < 0.05, **p < 0.01.