Table 2 Primers and details of PCRs used in the study
Gene(s) | Primer name | Primer sequence | Annealing temperatures and no. of cycles | Reference |
|---|---|---|---|---|
cytb and tRNAThr | DK14169F | 5′-CCACGACTARTGACACGAAAAATC-3′ | touchdown from 60 to 56°C, then 55°C for 30 cycles | this study |
|  | DK15396R | 5′-CAGCTTTGGGTGTTGATGGT-3′ |  | this study |
12S, tRNAVal, 16S | 12C | 5′-AAAGCAAARCACTGAAAATG-3′ | 50°C, 36 cycles | [92] |
|  | 12G | 5′- TTTCATCTTTTCCTTGCGGTAC-3′ |  | [92] |
rag1 | RAG1F1705 | 5′-GCTTTGATGGACATGGAAGAAGACAT-3′ | touchdown from 60 to 58°C, then 57°C for 33 cycles | [93] |
|  | RAG1R2864 | 5′-GAGCCATCCCTCTCAATAATTTCAGG-3′ |  | [93] |
rag2 | RAG2F220 | 5′-GATTCCTGCTA(CT)CT(TC)CCTCCTCT-3′ | touchdown from 60 to 58°C, then 57°C for 33 cycles | [93] |
|  | RAG2R995 | 5′-CCCATGTTGCTTCCAAACCATA-3′ |  | [93] |
nd1 | ND1F2 | 5′-GGCAGAGACCGGTAATTGCATAA-3′ | 52°C, 30 cycles | [37] |
|  | ND1R | 5′-GTATGGGCCCGATAGCTT-3′ |  | [37] |