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Table 2 Primers and details of PCRs used in the study

From: Hidden diversity in Senegalese bats and associated findings in the systematics of the family Vespertilionidae

Gene(s) Primer name Primer sequence Annealing temperatures and no. of cycles Reference
cytb and tRNAThr DK14169F 5′-CCACGACTARTGACACGAAAAATC-3′ touchdown from 60 to 56°C, then 55°C for 30 cycles this study
  DK15396R 5′-CAGCTTTGGGTGTTGATGGT-3′   this study
12S, tRNAVal, 16S 12C 5′-AAAGCAAARCACTGAAAATG-3′ 50°C, 36 cycles [92]
  12G 5′- TTTCATCTTTTCCTTGCGGTAC-3′   [92]
rag1 RAG1F1705 5′-GCTTTGATGGACATGGAAGAAGACAT-3′ touchdown from 60 to 58°C, then 57°C for 33 cycles [93]
  RAG1R2864 5′-GAGCCATCCCTCTCAATAATTTCAGG-3′   [93]
rag2 RAG2F220 5′-GATTCCTGCTA(CT)CT(TC)CCTCCTCT-3′ touchdown from 60 to 58°C, then 57°C for 33 cycles [93]
  RAG2R995 5′-CCCATGTTGCTTCCAAACCATA-3′   [93]
nd1 ND1F2 5′-GGCAGAGACCGGTAATTGCATAA-3′ 52°C, 30 cycles [37]
  ND1R 5′-GTATGGGCCCGATAGCTT-3′   [37]