Figure 6

Effects of acid stress on Na⁺accumulation and NCC expression in zebrafish larvae. (A, B): Comparison of whole body Na⁺ content (A) and ncc mRNA expression (B) in larvae treated with control pH7 (white bars) or acidic pH4 FW (gray bars) for 4 d. Mean ± SD (n = 6). (C, D): Immunostaining images of NCC (arrow) in 4-dpf larvae under pH7 or pH4 FW for 4 d. Scale bar = 100 μm. (E): Comparison of NCC cell number in different areas of 4-dpf larvae treated with pH7 (white bars) or pH4 FW (gray bars) (n = 8). *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t-test).