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Figure 1 | Frontiers in Zoology

Figure 1

From: Environmental monitoring using next generation sequencing: rapid identification of macroinvertebrate bioindicator species

Figure 1

Bootstrapped Kirma- 2-parameter trees examining the genetic distance between the species found in this study. Neighbour joining trees are based on the 46 chironomid species that occurred at the ten field sites for two gene regions a) COI b) CytB used in this study. Both trees are construct using the same regions used to identify species in the 454 pyrosequencing experiments (395 bps for COI and 343 bps for CytB) and show the level of intraspecific variation (represented by black triangles) based on sequences from up to ten individuals pre species (the number of individuals is given in parentheses) from our DNA reference libraries.

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