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Table 3 Performance of universal primer sets for COI across phyla

From: A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents

  Forward primer “mlCOIintF” “LCO1490” “dgLCO1490” “jgLCO1490” “Uni-MinibarF”
  Reverse primer “jgHCO2198” “HCO2198” “dgHCO2198” “jgHCO2198” “Uni-MinibarR1”
  Fragment length (bp) 313 658 658 658 130
Phylum Radiolaria (1) 0 0 0 0 0
Ciliophora (1) 0 1 0 0 0
Sarcomastigophora (1) 0 0 0 0 0
Amoebozoa (1) 0 0 0 0 0
Placozoa (1) 0 0 0 0 0
Porifera (4) 4 3 3 2 2
Cnidaria (28) 26 22 23 23 11
Ctenophora (2) 1 0 0 0 1
Chaetognatha (2) 2 1 2 2 0
Nematomorpha (1) 0 0 0 0 0
Nematoda (2) 1 1 0 0 0
Tardigrada (1) 0 0 0 0 0
Arthropoda (99) 87 84 80 82 30
Platyhelminthes (4) 4 1 1 0 0
Gastrotricha (3) 2 0 0 0 0
Gnathostomulida (3) 2 1 0 0 0
Rotifera (1) 1 1 1 0 0
Entoprocta (1) 0 0 1 0 0
Bryozoa (9) 9 9 8 7 5
Annelida (25) 25 23 25 23 5
Nemertea (4) 3 3 3 1 2
Sipuncula (5) 5 5 5 5 1
Mollusca (52) 47 45 49 48 11
Echiura (1) 1 1 1 1 0
Phoronida (2) 2 2 2 2 2
Brachiopoda (1) 0 1 1 1 0
Chordata (18) 15 9 12 12 4
Acoelomorpha (1) 0 0 0 0 0
Hemichordata (2) 2 0 1 2 1
Echinodermata (11) 11 4 2 11 1
Total (287) 250 217 220 222 76
   (87%) (76%) (77%) (77%) (27%)
  1. Columns present the number of taxa for which the target region was successfully amplified. Amplification success was evaluated on agarose gels (pictures shown in Additional file2). The total number of taxa used for each phylum is displayed in parentheses.