Table 3 Performance of universal primer sets for COI across phyla
Forward primer | “mlCOIintF” | “LCO1490” | “dgLCO1490” | “jgLCO1490” | “Uni-MinibarF” | |
|---|---|---|---|---|---|---|
Reverse primer | “jgHCO2198” | “HCO2198” | “dgHCO2198” | “jgHCO2198” | “Uni-MinibarR1” | |
Fragment length (bp) | 313 | 658 | 658 | 658 | 130 | |
Phylum | Radiolaria (1) | 0 | 0 | 0 | 0 | 0 |
Ciliophora (1) | 0 | 1 | 0 | 0 | 0 | |
Sarcomastigophora (1) | 0 | 0 | 0 | 0 | 0 | |
Amoebozoa (1) | 0 | 0 | 0 | 0 | 0 | |
Placozoa (1) | 0 | 0 | 0 | 0 | 0 | |
Porifera (4) | 4 | 3 | 3 | 2 | 2 | |
Cnidaria (28) | 26 | 22 | 23 | 23 | 11 | |
Ctenophora (2) | 1 | 0 | 0 | 0 | 1 | |
Chaetognatha (2) | 2 | 1 | 2 | 2 | 0 | |
Nematomorpha (1) | 0 | 0 | 0 | 0 | 0 | |
Nematoda (2) | 1 | 1 | 0 | 0 | 0 | |
Tardigrada (1) | 0 | 0 | 0 | 0 | 0 | |
Arthropoda (99) | 87 | 84 | 80 | 82 | 30 | |
Platyhelminthes (4) | 4 | 1 | 1 | 0 | 0 | |
Gastrotricha (3) | 2 | 0 | 0 | 0 | 0 | |
Gnathostomulida (3) | 2 | 1 | 0 | 0 | 0 | |
Rotifera (1) | 1 | 1 | 1 | 0 | 0 | |
Entoprocta (1) | 0 | 0 | 1 | 0 | 0 | |
Bryozoa (9) | 9 | 9 | 8 | 7 | 5 | |
Annelida (25) | 25 | 23 | 25 | 23 | 5 | |
Nemertea (4) | 3 | 3 | 3 | 1 | 2 | |
Sipuncula (5) | 5 | 5 | 5 | 5 | 1 | |
Mollusca (52) | 47 | 45 | 49 | 48 | 11 | |
Echiura (1) | 1 | 1 | 1 | 1 | 0 | |
Phoronida (2) | 2 | 2 | 2 | 2 | 2 | |
Brachiopoda (1) | 0 | 1 | 1 | 1 | 0 | |
Chordata (18) | 15 | 9 | 12 | 12 | 4 | |
Acoelomorpha (1) | 0 | 0 | 0 | 0 | 0 | |
Hemichordata (2) | 2 | 0 | 1 | 2 | 1 | |
Echinodermata (11) | 11 | 4 | 2 | 11 | 1 | |
Total (287) | 250 | 217 | 220 | 222 | 76 | |
(87%) | (76%) | (77%) | (77%) | (27%) |