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Table 2 Preliminary tests to determine the primer combination that performed best to amplify a short COI fragment

From: A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents

  Forward primer mlCOIintF LCO1490 dgLCO1490 jgLCO1490
  Reverse primer HCO2198 dgHCO2198 jgHCO2198 mlCOIintR
  Fragment length (bp) 313 313 313 319 319 319
Phylum Cnidaria (6) 6 6 6 2 2 2
Arthropoda (18) 16 15 16 12 11 11
Rotifera (1) 1 1 1 0 0 0
Entoprocta (1) 0 0 0 1 1 0
Annelida (4) 4 4 4 3 4 4
Nemertea (2) 2 2 2 0 0 1
Mollusca (9) 7 7 8 7 7 7
Echiura (1) 1 1 1 1 1 1
Chordata (2) 2 2 2 1 2 1
Hemichordata (2) 2 2 2 0 0 2
Echinodermata (1) 1 1 1 0 0 1
TOTAL (47) 42 41 43 27 28 30
   89% 87% 91% 57% 60% 64%
  1. Columns show the number of taxa for which the target region was successfully amplified. The total number of taxa used for each phylum is displayed in parentheses.