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Table 2 Preliminary tests to determine the primer combination that performed best to amplify a short COI fragment

From: A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents

 

Forward primer

mlCOIintF

LCO1490

dgLCO1490

jgLCO1490

 

Reverse primer

HCO2198

dgHCO2198

jgHCO2198

mlCOIintR

 

Fragment length (bp)

313

313

313

319

319

319

Phylum

Cnidaria (6)

6

6

6

2

2

2

Arthropoda (18)

16

15

16

12

11

11

Rotifera (1)

1

1

1

0

0

0

Entoprocta (1)

0

0

0

1

1

0

Annelida (4)

4

4

4

3

4

4

Nemertea (2)

2

2

2

0

0

1

Mollusca (9)

7

7

8

7

7

7

Echiura (1)

1

1

1

1

1

1

Chordata (2)

2

2

2

1

2

1

Hemichordata (2)

2

2

2

0

0

2

Echinodermata (1)

1

1

1

0

0

1

TOTAL (47)

42

41

43

27

28

30

  

89%

87%

91%

57%

60%

64%

  1. Columns show the number of taxa for which the target region was successfully amplified. The total number of taxa used for each phylum is displayed in parentheses.
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Frontiers in Zoology

ISSN: 1742-9994