Figure 4

Nanaloricus sp. Light micrography of an adult stage (A). Body musculature visualized by fluorescently-labeled phalloidin staining and CLSM (B) and by 3D imaging (C-D). Anterior faces upwards in all aspects. A: Male specimen with introvert retracted. B: Ventral view. C: Ventral view. D: Dorsal view. In this specimen the neck circular muscle merges the net-like thin muscles in the head. The pharyngeal bulb musculature is located posteriorly within the abdominal cavity. Color code: abdominal transverse muscle I, red; abdominal transverse muscles II-V, light blue; buccal tube retractors and mouth cone retractors, white; buccal tube ring muscle, dark red; dorsal longitudinal retractor muscle, dark blue; introvert anterior ring muscle, dark red; introvert posterior ring muscle, brown; neck circular muscle, purple; net-like thin muscles, dark yellow; pharyngeal bulb musculature, yellow; short posterior longitudinal muscles, green; posterior short curved muscles, light purple; thin longitudinal muscles, dark green; ventral longitudinal retractor muscle, orange. Abbreviations: *, pharyngeal bulb musculature; an, anus; at_I-V, abdominal transverse muscles; bu, buccal tube; cs, clavoscalid; dl, dorsal longitudinal retractor muscle; lo, lorica; mc, mouth cone; mcr, mouth cone retractors; nc, neck circular muscle; nl, net-like thin muscles; pa, pre-pharyngeal apparatus; pb, pharyngeal bulb; ss, spinoscalid; sp, short posterior longitudinal muscles; te, testis; tl, thin longitudinal muscles; vl, ventral longitudinal retractor muscle; arrows, posterior short curved muscles; arrowheads, introvert posterior ring muscle; double arrows, posterior thin fibers; double arrowheads, buccal tube ring muscle.