Phylogenetic tree (a) based on AFLP-data and chronogram (b) based mtDNA data of all Barombi Mbo cichlids (inside blue box), reference specimens of the closest riverine ancestor of the Barombi flock, Sarotherodon galilaeus, and two undescribed Sarotherodon from Lake Ejagham (S. sp. "bighead" and S. sp. "mudfeeder"). Photographs refer to the taxon to the left, different populations of S. galilaeus are depicted by two identical photographs only. (a) Numbers at nodes in the AFLP-tree are bootstrap-values (%) of tree reconstructions using the pruned (above) and unpruned (below) AFLP data set. Topologies were identical except for the position of S. g. "Niger", which was sistergroup to S. g. multifasciatus when using the unpruned data (bootstrap value: 86). Long terminal branches in the original phylogram were cut to identical lengths for graphical reasons; interior branch lengths are as in the original phylogram. (b) Numbers at nodes in the mtDNA-chronogram refer to bootstrap-values of the ML (above) and MP (below) tree reconstructions. The absolute time scale above the tree is based on the maximum age of the Barombi Mbo crater formation of 1.0 mya . Encircled numbers mark nodes referring to phylogenetic groups tested with PCO (see Table 1). Red, blue and green shaded boxes unite hypothetical ancient syngameons as deduced from the conflict between mtDNA- and AFLP-based phylogenetic hypotheses. Further details of tree reconstruction see Methods.