Figure 5

(A-C): DiI-labeling of cells within epithelial vesicles. Flat preparation of the fourth prosomal hemi-neuromere of an embryo labeled with DiI (red) and stained with phalloidin-rhodamine (green). (A-C) Invaginated cells in 40 segments of 10 embryos were labelled with DiI (red) and stained with phalloidin-FITC (green). The cells of a group (A, asterisks) are attached to each other (B,C large arrow head) and their short, thin cell processes run parallel to each other (B,C arrow). They do not show any morphological features of differentiation, i.e. they do not grow long thin dendritic or axonal processes. The small arrows (B,C) point to a cell of an adjacent invagination group.