Fig. 2

Early embryonic ectoderm matures into two distinct tissues. A Sorting according to ectodermal patterning within the transcriptomic data is evidenced by comparing expression patterns known from in situ hybridization with expression in the dimensional reduction. B Schematic illustrates the locations of the ectodermal layers in the adult. UMAP dimensional reduction of the ectodermal partition colored by cell state identity highlighting the retention of both spatial and temporal organisation within the clustering. C Relative distribution of cell cluster identities across all samples in the dataset. E Dotplot representation of differentially expressed gene sets from all genes (left) and restricted to transcription factors only (right). Expression profiles are split between the two subsets. Orange scale = Adult subset; Dark slate blue scale = Developmental subset