Effects of reduced kinematic and social play experience on affective appraisal of human-rat play in rats

Table 3 Factor level analysis of the fixed effects in the best models

Fixed effects	Sum of squares	F value	p Value
(a) 50-kHz USV best model
Treatment	6454	1.72	0.19
Days	32,336	17.32	< 0.001
Order	10,248	5.49	0.03
Batch	2418	0.65	0.22
b) 22-kHz USV best model
Days	427.87	427.87	< 0.001
Batch	31.29	15.65	< 0.001
c) USV emission by contact periods best model
Treatment	0.62	1.14	0.35
Contact periods	182.27	338.17	< 0.001

Marginal and conditional r² for the best 50-kHz USV model are 0.14 and 0.70, for the best 22-kHz USV model are 0.34 and 0.96, for the best USV emission by contact periods model are 0.40 and 0.72. Statistically significant effects are in bold

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