Fig. 4
Sub-slice imaging (optical sectioning). A Layers 50 nm thick were cut using a diamond knife and two primary beam energies (2.5 kV and 4 kV) were used to scan information from layers 25 nm apart (scheme). Single scans of cytoplasm and mitochondria in a transporting epithelium (images). Volume data from this area are in the movie in Additional file 8. B–E High-resolution trial datasets. Volume reconstruction of muscle and adjacent tissue (B, D) and gut tissue (C, E) showing cross-sections in all three axes (B, C) and volume rendering (D, E). Data was acquired by a combination of physical (40 nm) and virtual (10 nm) slicing. The voxel size was 8 × 8 × 10 nm3 with a volume of 16 × 12 × 13 μm3 (B, D) and isometric voxel 10 × 10 × 10 nm3 with a volume of 23 × 13 × 12 μm 3 (D, E). Movies for B, D and C, E are in Additional files 9 and 10, respectively. Samples: A O. cincta—sample 1, B, C A. dispar—sample 3, D, E O. cincta—sample 6. (Additional file 6). cr, cristae in mitochondria; nu, nucleus; res, resin. Arrowhead marks a nucleopore. Scale bars: 5 μm