Fig. 3
Examples of SBF-SEM images acquired from MR embedded samples. Single scans of the sample surface, several tiles stitched together. A–A″ Less successfully embedded sample, where too much resin (res) around it remained. Low vacuum mode, 20 Pa. B–E′ Resin sufficiently removed. High vacuum mode. Samples (details in Additional file 6): A–A″ C. franzi—sample 6 (first abdominal appendage—longitudinal section); B O. cincta—sample 1 (collophore—cross section); C O. cincta—sample 2 (trunk body wall); D O. latipes (whole roe); E, E′ A. dispar—sample 5 (proximal part of the first abdominal appendage, neighboring muscles). Arrowheads mark residual charging around the sample (black regions in these inverted images). go, gold sputter coating; lys, lysosome; mu, muscle; nu, nucleus; rer, rough endoplasmic reticulum; res, resin. Scale bars: A–A″, C–E′, 5 μm; B, 10 μm