Visual acuity and contrast sensitivity of adult zebrafish
© Tappeiner et al.; licensee BioMed Central Ltd. 2012
Received: 27 December 2011
Accepted: 12 May 2012
Published: 29 May 2012
The aim of this study was to evaluate the visual acuity of adult zebrafish by assessing the optokinetic reflex. Using a modified commercially available optomotor device (OptoMotry®), virtual three-dimensional gratings of variable spatial frequency or contrast were presented to adult zebrafish. In a first experiment, visual acuity was evaluated by changing the spatial frequency at different angular velocities. Thereafter, contrast sensitivity was evaluated by changing the contrast level at different spatial frequencies.
At the different tested angular velocities (10, 15, 20, 25, and 30 d/s) and a contrast of 100%, visual acuity values ranged from 0.56 to 0.58 c/d. Contrast sensitivity measured at different spatial frequencies (0.011, 0.025, 0.5, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.55 c/d) with an angular velocity of 10 d/s and 25 d/s revealed an inverted U-shaped contrast sensitivity curve. The highest mean contrast sensitivity (±SD) values of 20.49 ± 4.13 and 25.24 ± 8.89 were found for a spatial frequency of 0.05 c/d (angular velocity 10 d/s) and 0.1 c/d (angular velocity 25 d/s), respectively.
Visual acuity and contrast sensitivity measurements in adult zebrafish with the OptoMotry® device are feasible and reveal a remarkably higher VA compared to larval zebrafish and mice.
KeywordsAdult zebrafish Visual acuity Optokinetic reflex Optomotry
Zebrafish (Danio rerio), which were initially used as an animal model in developmental biology due to high fecundity and ease of maintenance, has found its way into visual research due to its excellent visual system with a cone-dominated retina . In the past, measurements of visual functions have been performed in larval zebrafish . Furthermore, visual acuity (VA) of larval zebrafish has previously been determined by assessing the optokinetic reflex (OKR) in a self-constructed set-up . Certain visual functions of adult zebrafish (gain of eye velocity at different spatial frequencies and contrast levels) have been published [4–6]. However, no empirical data about VA of adult zebrafish are available up to now. A standardized and reliable method for VA measurements is a prerequisite to analyze genetically modified fish lines and to evaluate the effect of a therapeutic action (e.g. impact of drugs or genetic interventions on the visual system) in addition to histological and ophthalmological examination of adult zebrafish . OKR measurements have the advantage of a stereotyped and robust behavior and no prior training of the animal is necessary. The OKR consists of a slow movement in the direction of a perceived motion, followed by a quick return movement, called saccade . Due to the relatively big eyes, the OKR of zebrafish can easily be detected. Therefore, the OptoMotry device was used for this study as it allows standardized OKR measurements.
OKR measurements are performed presenting moving gratings to the animal, and assessing reflexive responses, which are based on subcortical circuits. This technique allows to determine the spatial frequency threshold and constitutes one possibility to measure visual acuity. When referring to VA measurements based on OKR assessment, e.g. with the OptoMotry system, the term “visual acuity” corresponds to “spatial frequency threshold”. For simplicity and for the comparison to previous publications, only the term “visual acuity” is used in this manuscript.
The aim of the present study was to evaluate the VA and contrast sensitivity of adult zebrafish based on OKR assessments with a modified, commercially available and standardized system (OptoMotry).
Materials and methods
Wild-type zebrafish (Danio rerio) of the AB (Oregon) strain, aged between 12 and 24 months, have been used. Experiments were performed at room temperature (approximately 20° Celsius). To facilitate the placement of the fish in the examination chamber, fish were shortly sedated in 0.1% Tricaine (Sigma-Aldrich, Buchs, Switzerland). Then, fish were irrigated with fresh water and were awake during the following experiment. Experimental research on animals has been approved by the governmental authorities and adhered to the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research.
The zebrafish was positioned in an examination chamber, which was designed to allow a constant flow of fresh water (Figure 1b). The hydrostatic pressure of an infusion bottle, placed 100 to 120 cm above the examination chamber, generated the necessary flow, which was adjustable by regulating the infusion valve. The examination chamber (L x W x H = 40 x 14 x 10 mm) was constructed with transparent polystyrene. The head of the zebrafish was located in the cylinder-shaped end of the chamber (R = 14 mm), allowing the animal an unhindered view to the sine wave gratings shown on the screens of the optomotor system. Inside the examination chamber, the animal was placed on a platform positioned 13 cm above the floor. Eye movements were recorded with a Sony DCR-HC26 Handycam®.
Additional file 1 : Video S1. Top view of an adult zebrafish in the examination chamber displaying the optokinetic reflex (OKR), which consists of a slow movement in the direction of a perceived motion, followed by a quick return movement. The direction of the OKR can be easily assessed. First, stationary gratings are presented to the fish, followed by well visible moving gratings (spatial frequency 0.05 c/d; contrast 100%; angular velocity 30 d/s). At the end, stationary gratings are again presented to the fish. The red stripes have been added to the video to simulate the direction of the grating. (MP4 8 MB)
Afterwards, contrast sensitvitiy was evaluated by changing the contrast level (stair-case procedure) at different spatial frequencies (0.011, 0.025, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5 and 0.55 c/d) until the zebrafish no longer responded with an OKR. Experiments were performed at an angular velocity of 10 and 25 d/s.
To compare results of the OptoMotry device to according data determined with another set-up , we have also measured larval zebrafish. A drop of water was placed on a plastic plate on the platform of the OptoMotry system. Then the larva was put into this drop of water. A Volk 2.2 lens was mounted 3.5 cm above the larva to allow an adequate magnification for the observation of the animals using the video system of the OptoMotry device (total magnification factor, including optical and digital zoom of the camera, of approximately 15x). An angular velocity of 15 d/s was chosen for direct comparison of the results to previously published data .
In order to extend VA measurements with the OptoMotry system to another species, also medaka (Oryzias latipes), aged 3 to 4 months, have been measured. An angular velocity of 15 and 25 c/d was chosen (contrast of 100%). The experimental procedure was analogue to the one in adult zebrfish. Since the size of adult zebrafish and medaka is similar, it was possible to use the same experimantation chamber.
The statistical analysis was performed using a Friedmann test with Dunns post-hoc analysis. A p-value < 0.05 was considered significant.
Mean visual acuity and standard deviation (SD) for different angular velocities in adult zebrafish (3 experiments with independent observers and n = 6 zebrafish, each) at a contrast of 100%
Angular velocity (d/s)
Mean visual acuity ± SD (c/d)
0.563 ± 0.032
0.578 ± 0.021
0.586 ± 0.016
0.589 ± 0.015
0.584 ± 0.019
Mean contrast sensitivity and standard deviation (SD) for tested spatial frequencies (0.011 – 0.55 c/d) at two different angular velocities (10 d/s and 25 d/s) (2 experiments with independent observers and n=6 zebrafish, each)
Spatial frequency (c/d)
5.63 ± 1.66
6.12 ± 1.94
9.66 ± 1.75
12.97 ± 2.47
20.49 ± 4.13
24.91 ± 8.08
15.51 ± 5.83
25.24 ± 8.89
7.28 ± 2.64
16.24 ± 8.41
4.14 ± 1.53
9.70 ± 6.19
1.79 ± 0.24
5.27 ± 3.74
1.17 ± 0.11
1.66 ± 0.60
1.04 ± 0.04
1.10 ± 0.11
For larval zebrafish, a mean VA of 0.16 ± 0.016 c/d was determined at an angular velocity of 15 d/s and a contrast of 100%. Adult medaka revealed a mean VA of 0.53 ± 0.054 c/d at an angular velocity of 15 d/s and and a VA of 0.56 ± 0.045 c/d at an angular velocity of 25 d/s (contrast of 100%, each).
Visual acuity, defined as the ability to distinguish two objects (spatial resolution) at a given angular distance, is limited by different factors of the visual system, e.g. dimensional and optical properties of the eye, neural properties of the retina and visual processing in higher brain centers. Particularly, the distance between photoreceptor cells plays a pivotal role . Based on photoreceptor spacing and optical properties of the eye, Haug et al. calculated a maximum possible VA of 0.24 c/d in 5-day old larval zebrafish and of 0.871 c/d in adult zebrafish . This difference in VA is mainly explained by the different eye size: due to geometrical reasons a larger eye leads to a larger retinal image with better VA as photoreceptor spacing is comparable between the two development stages .
Most VA measurement techniques available for animal experiments [10–14] require prior training of the animal and are time-consuming. In contrast, VA testing methods based on an innate behavior need no prior training of the animal. In mice and rat, VA can be measured using the optomotor reflex . Briefly, a virtual rotating cylinder with vertical sine wave grating provokes reflexive head and neck movements. Like the optomotor reflex, also the OKR is based on innate reflexive behavior. The OKR can be repeatedly provoked independently of the animal’s cooperation and their large eyes can easily be observed, which makes assessment of the OKR an ideal method for VA measurements in zebrafish.
As larval zebrafish absorb enough oxygen through their skin, they can be embedded in methylcellulose for VA measurements . This approach is not feasible in adult zebrafish, which need a constant flow of oxygenated water irrigating their gills. Restricting body movements in the adult zebrafish was rather challenging, we did however solve this problem by designing a custom made flow through chamber. It was not necessary to fixate the body of the fish in our experiments, as the small chamber offered enough restriction to allow the observation of eye movements. This is likely less incriminating to the fish. However, a fixation would still be possible with the provided set-up (for instance by clamping the body of the anesthetized fish between two pieces of sponge ) if necessary for certain experiments.
With optimal settings, we determined a best mean VA of 0.59 c/d in adult zebrafish and a best mean VA of 0.16 c/d in larval zebrafish. The latter is equal to the mean VA of larval zebrafish in a previous study using another set-up . The VA of adult zebrafish is higher than the mean VA measured in larval zebrafish but lower than the mathematically one estimated by Haug et al. . The ratio of the measured and the calculated VA was similar for larval and adult zebrafish (0.67 for larval and 0.68 for adult zebrafish). It may be assumed that the theoretically possible VA in the mathematical model is not achievable due to optical imperfections. On the other hand, VA values also depend on measurement techniques, e.g., in humans the VA obtained by subjective testing with a visual acuity chart is known to be higher than the one obtained by OKR measurements [15, 16].
To exemplarily demonstrate that the OptoMotry device can also be used to determine VA in other species, we have tested medaka with the same standardized set-up. The adult fish of both species, zebrafish and medaka, revealed a comparable VA.
Contrast sensitivity is an important parameter of vision. In humans, the impairment of contrast sensitivity can precede loss of visual acuity and is related to functional disability . The relation of contrast sensitivity and spatial frequency can be depicted as a contrast sensitivity curve. In adult zebrafish a typical inverted U-shaped contrast sensitivity curve with a fall off in sensitivity at low and high spatial frequencies can be found which is also seen in other species including humans [9, 18, 19].
A similar peak contrast sensitivity as in adult zebrafish has been found in adult mice, also using the OptoMotry device . In humans, contrast sensitivity is significantly higher . On the other hand, contrast sensitivity of adult zebrafish is considerably higher than in larval zebrafish  and birds . Overall, adult zebrafish reveal quite a good contrast sensitivity, which might be an adaption to their natural habitat of shallow waterbodies with silty substrate .
Measurement of VA and contrast sensitivity based on the OKR is feasible in adult zebrafish, revealing a remarkable higher VA compared to larval zebrafish and mice [3, 12]. As the OptoMotry device is commercially available, standardized and reproducible measurements are possible. We believe that the presented set-up does not only allow screening for adult vision mutants but will also help to better analyze the vision of wild-type or mutant zebrafish and pharmacologically induced disease models.
We thank Ying-Yu Huang and Bettina Rotzetter for the critical review of the manuscript, Monika Kilchenmann for her technical assistance and Gerrit Begemann for providing the medaka. This research was supported by an unrestricted grant from the Berne University Research Foundation.
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