Effects of dietary sodium on performance, flight and compensation strategies in the cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)
© Xiao et al; licensee BioMed Central Ltd. 2010
Received: 17 August 2009
Accepted: 13 April 2010
Published: 13 April 2010
Sodium is critical for many physiological functions in insects. Herbivorous insects should expend considerable energy to compensate for sodium deficiency due to low sodium concentration in most inland plants upon which they feed. However, sodium compensation behaviors such as mud-puddling have been observed in some species but not in others. We expect that there may be other sodium compensation strategies in insects. Here, we select a rarely mud-puddling insect species, the cotton boll worm, Helicoverpa armigera, and determine the effects of dietary sodium on performance and flight, and examine their means of sodium compensation.
When freshly hatched H. armigera neonates were cultured on one of three diets differing in sodium contents (diet A, B and C with a high, middle and low sodium concentrations, respectively), the larvae on diet C grew larger, had a higher mortality rate and a shorter development period than those on diet A and B. The larvae previously fed from 1st to 3rd instar on diet C consumed more subsequent diet when they were transferred to diet A or C at 4th instar, comparing to those previously fed on diet A. Moreover, any 4th-instar larvae on diet C consumed a greater amount of food than those on diet A, no matter which diet the larvae had previously ingested from 1st to 3rd instar. Moths from diet A and B flew more rapidly than those from diet C, with similar sugar and lipid utilization rates among the three test groups. When a 5th-instar cannibal from diet A, B or C and a 5th-instar victim from diet A were housed together, many more cannibals from diet C ate their victims. When a victim from diet A, B or C was provided, a cannibal from diet C was more likely to eat the victim from diet A. When newly emerged moths had been exposed to 3% sodium chloride solution for all scotophase period, the average weight increase (proxy for sodium solution intake) for moths from diet A was lower than those from diet B or C.
Sodium-deficient diet resulted in rapid growth and development of H. armigera larvae, decreased larvae survival, and reduced flight speed of H. armigera adults. To compensate for sodium deficiency, H. armigera ingested a large quantity of larval food, increased larval cannibalism incidence and harvested sodium during the adult stage.
Sodium plays important roles in osmotic balance, in neuromuscular system, and in digestion and excretion process in animals [1–4]. Therefore, animals have a dietary sodium requirement [5–7]. Moreover, body sodium is tightly regulated in some animals [6, 8].
Sodium concentration is very low in most inland plants [9–12]. In contrast, the herbivores that eat them maintain much higher sodium levels. In vertebrates, sodium concentration was 100- to 1000-fold more than that in plants . In insects, the sodium concentrations in the haemolymph of phytophages in Orthoptera, Phasmida and Lepidoptera were much higher than that in plants. Moreover, the adult haemolymph of most holometabolous insects had higher sodium concentrations than that of the larvae. In the Lepidopterans Mamestra brassicae and Bombyx mori, for example, the sodium concentration in the adult blood was 3 and 8 times more than that in the larvae blood, respectively . It seems that herbivores must expend considerable energy to find and harvest sodium [3, 4, 14–16].
Many vertebrates (cattle, chamois, cow, elk, deer, goat, gorillas, kangaroos, moose, parrots, rabbit, sheep, and stags) have been documented to exhibit an appetite for salt in nature [9, 15, 17–19]. In invertebrates, some butterflies [20–30], moths [21, 31–33], honeybees , ants , cicadellids  and Ceracris kiangsu adults  searched for potential salt sources such as moist ground, perspiration, tears, excrements, or rotten fish. This phenomenon was defined as mud-puddling and some of these insect species, such as the notodontid moth, Gluphisia septentrionis, have been proven to mud-puddle to collect sodium .
Interestingly, mud-puddling behaviors were often observed in some insect species but rarely or never found in others. If sodium deficiencies are common in herbivores [10, 36–40], one can expect that non-puddling insect herbivores have other sodium compensation strategies.
Arms et al.  and Downes  suggested that sodium might be important for maintaining high neuromuscular activity. In Riodinidae, 124 species in 41 genera were collected and male feeding behavior and morphology relationship was analyzed . Among these species, fish carrion feeders and puddlers had lower mean wing area to thoracic volume ratios (WA:TV) than flower nectarers. Hall and Willmott  found that WA:TV ratio was significantly negatively correlated with flight speed and oxygen consumption (a direct indicator of metabolic rate), and suggested that fish carrion feeders and puddlers may obtain some substances to provide the necessary nutrients to maintain high metabolic rates during rapid flight. Proving the sodium content in fish (0.6 mg/g) is high, this result indicates that sodium is the most possible candidate for fish carrion feeders and puddlers in Riodinidae to maintain rapid flight. The results reported by Molleman et al. , however, did not support the indication. They found sodium concentration in the species with the smallest WA:TV ratio was relatively low . Therefore, we need further research to confirm the effect of sodium on flight speed and flight duration.
The cotton bollworm, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) continues to challenge crop production throughout much of its distribution in tropical and subtropical region of the Old World [41, 42]. The adults are hardly found to mud-puddle. Here, we varied the sodium concentrations in a H. armigera larval artificial diet to obtain resulting larvae, pupa and adults and addressed the following questions. (1) How does dietary sodium influence larvae performance and adult flight? and (2) How does H. armigera compensate for sodium deficiency?
H. armigera larvae were collected from cotton plants (Gossypium hirsutum) at Nanjing (32.0N, 118.5E), Jiangsu Province in China in July and August 2008 and routinely reared in an insectary under controlled temperature (28 ± 1°C), photoperiod (14 h light/10 h dark) and relative humidity (70-80%) until pupation. Females and males were then segregated based on the morphology of the abdominal terminal segments of the pupa. Newly emerged moths were given unlimited access to a 10% sucrose solution, and allowed to mate for two successive nights. Eggs were collected, sterilized and transferred to a 1000 ml glass pot according to the method of Xu et al.  and Liu et al. . Between 12 and 24 h prior to egg hatching, an artificial diet was offered. The diet contained wheat-germ powder (27.5 g), soybean flour (7.5 g), yeast (2.0 g), agar (2.5 g), water (93 ml), Wesson salt mixture (0. 2 g) , vitamins (vitamin C 0.7 g, B1 0.41 mg, B2 0.82 mg, B6 0.41 mg, B12 0.01 mg, biotin 0.04 mg; nicotinamide 1.63 mg, folic acid 0.41 mg; calcium pantothenate 1.63 mg), inositol (32.64 mg) and citric acid (0.2 g). When larvae developed to the late second instar, they were transferred individually to a 15 ml glass vial containing a piece of the artificial diet until pupation.
To estimate the effects of dietary sodium on performance, flight and compensation strategies in H. armigera larvae or adults, three larval diets differing in sodium concentrations were prepared. Diet A was a conventional diet. Diet B was modified from the conventional diet in which half amounts of sodium chloride (NaCl) and sodium fluoride (NaF) in Wesson salt mixture were replaced with equal mole of potassium chloride (KCl) and potassium fluoride (KF), respectively. Diet C was modified from the conventional diet in which all NaCl and NaF in Wesson salt mixture were replaced with equal mole of KCl and KF. After dried and ashed in a muffle furnace, the concentrations of sodium and potassium in the three diets were determined by an atomic absorption spectrophotometer. The average concentration of sodium in diet A, B and C is 0.43 ± 0.013, 0.30 ± 0.010 and 0.14 ± 0.005 mg/g dry weight (n = 5), and that of potassium is 3.12 ± 0.094, 3.47 ± 0.134, and 3.78 ± 0.143 mg/g dry weight (n = 5), respectively. Between 12 and 24 h prior to egg hatching, one of the three diets was offered. The resulting larvae, pupa or adults were used in experiments.
Observations of growth parameters were made for the larvae that were cultured on the three different diets. Survival rate from 1st to 3rd instar, body weight increase, development duration, the rate of larvae to reach the pupal stage (pupation rate), pupa weight, emergence rate, adult weight, and percent of moth with abnormal wing shape were determined. For each test diet 30 to 64 individuals were observed and repeated three times.
To determine the effect of previous food intake on subsequent food consumption by larvae, the larvae previously fed on diet A from 1st to 3rd instar, and then the resulting 4th-instar larvae were transferred to diet A or C, respectively. Likewise, those previously fed on diet C from 1st to 3rd instar were also split into two groups, one was provided with fresh diet C, and the other with diet A. The diet weight was measured before transfer and 24 h after the transfer. Since our preliminary measure showed that the artificial diet in a 15 ml glass vial lost little water over a 24 h period, the consumption amount of subsequent food by each larva was calculated as the difference between the two weights.
To test sodium harvest by adults, one-day-old moths originated from diet A, B or C were introduced individually into a 100 ml jar, given unlimited access to a 3% sodium solution during scotophase, a 10 h dark phase from 8:00 PM to 6:00 AM. The body weights of the moths were weighed before and after scotophase, and the average weight increase was proxy for sodium solution intake.
Sodium chloride (NaCl), sodium fluoride (NaF), potassium chloride (KCl), potassium fluoride (KF), potassium iodide (KI), potassium dihydrogen phosphate (KH2PO4), alum (KAl(SO4)2·12H2O), calcium phosphate (Ca3(PO4)2), calcium carbonate (CaCO3), ferric phosphate (FePO4), magnesium sulfate (MgSO4), cupric sulfate (CuSO4), and manganese sulfate (MnSO4) were purchased from Shantou Xilong Chemical Factory and were mixed of the composition in Wesson  to make Wesson salt mixture. Vitamin C, B1, B2, B6, B12, biotin, nicotinamide, folic acid, calcium pantothenate, inositol, and citric acid were obtained from Sigma (St. Louis, MO). Anthrone (C14H10O), sulfuric acid (H2SO4), and trichloroacetic acid (C2HCl3O2) were from Sinopharm Chemical Reagent Co. Ltd. Ethanol (C2H5OH), ethyl acetate (C4H8O2), glucose (C6H12O6), and ethyl ether (C4H10O) were from Shanghai Experiment Reagent Co. Ltd. All had claimed purities of 99%.
Cannibalism was observed by a method similar to Simpson et al. . The 5th-instar larvae fed on diet A, B or C were selected as cannibals or victims. The victims were freshly incapacitated by rupture of the head capsule to make them can not injure cannibals. A cannibal and a victim were housed in a culture dish (12 cm in diameter and 2 cm in height). Each treatment was replicated 20 to 30 times. A victim was classified as cannibalized if body parts were either partially or entirely missing. One hour later the cannibalism incidences were counted.
Tethered flight test
All flight tests were done with a computer-interfaced tethered flight system similar to that of Naranjo  and Murata and Tojo . Each one-day-old moth (24 h after emergence) was anesthetized under carbon dioxide (exposure time was about 1 min), and the scales on the back of thorax were removed using a small brush. The moth was then anesthetized under carbon dioxide and adhered to one end of a lightweight lever, which was balanced by a small flag attached to the opposite end and was pivoted on a fulcrum. After recovery from anesthesia, the moth was allowed to fly and the lever rotated around the fulcrum. The rotation cycles of the lever were recorded and the flight duration and distance for each moth over a 24-h dark phase were calculated by the computer.
Total carbohydrates and lipid analyses
Before or after flight, the male and female moths were frozen and lyophilized at -20°C. The moths were weighed, and the total lipid was extracted with chloroform-methanol (2:1 v/v) according to Folch et al.  and total carbohydrates were extracted with boiling water. Total lipid was determined by the phospho-vanillin method as described by Ayali and Pener  and total carbohydrate was tested by the anthrone method .
The data were given as means ± SE. Statistical tests included one-way, repeated measures and 2 × 2 factorial ANOVAs. A repeated measures ANOVA was used to test for effects of different diets and feeding days on larval growth. A 2 × 2 factorial ANOVA was used to test for effect of previous food intake on subsequent food consumption by larvae, with 1st to 3rd instar pre-treatment diet as the first factor and the subsequent diet as the second factor. The differences among treatments were compared by Duncan's New Multiple Range Test . Differences in cannibalism incidences were compared by a chi-square test.
Effects of diet on growth of the larvae
A repeated-measures ANOVA for effect of diet and larvae age (days after hatching) on larvae body weight
Larvae age × Diet
The mean body weights of the H. armigera larvae feeding on diet A, B and C
Larvae fed on
Larvae body weight (mg) *
21.1 ± 0.3
36.5 ± 1.2
85.8 ± 2.3
122.2 ± 5.4 d
211.5 ± 9.6 B
20.9 ± 0.4
35.7 ± 1.0
86.6 ± 2.0
134.6 ± 6.0 Cd
226.3 ± 7.2 B
21.8 ± 0.3
39.0 ± 1.2
98.4 ± 2.0
140.9 ± 5.0 C
257.9 ± 8.1 A
The larval survival rate from 1st to 3rd instar of the three test groups fed on diet A, B and C was 85.6 ± 1.7%, 70.4 ± 2.1%, and 65.6 ± 1.9% respectively, the former was significantly higher than the latter two. Development time of the three test groups on diet A, B and C was 14.9 ± 0.13 days, 14.8 ± 0.13 days and 14.4 ± 0.14 days, respectively, the former two developed significantly slower than the latter. The pupation rates (93.8 ± 8.4%, 91.7 ± 7.7% and 92.7 ± 8.3%, respectively) and pupa weights (302.7 ± 13.3 mg, 311.7 ± 9.3 mg and 298.9 ± 11.8 mg, respectively) of the three test groups on diet A, B and C showed little differences.
Effects of previous diet intake on subsequent food consumption
Effects of larval diet on the adults
Influences of larval diets on the H. armigera adult emergence and body weight
Larvae fed diet
Percent of moth with
abnormal wing shape*
Body weight (mg)*
83.5 ± 5.9 a
8.3 ± 0.6 b
141.4 ± 5.2 a
86.2 ± 4.6 a
10.5 ± 0.8 b
133.9 ± 3.6 ab
84.3 ± 6.1 a
14.5 ± 0.9 a
126.5 ± 4.0 b
84.1 ± 8.7 a
9.5 ± 0.6 b
158.6 ± 3.1 a
84.8 ± 5.7 a
11.1 ± 0.7 ab
139.2 ± 5.5 b
84.9 ± 7.4 a
14.5 ± 1.2 a
138.0 ± 7.2 b
Effects of larval diets on flight capacity in the adults
Effects of larval diets on flight capacities in the adults
Larvae fed on diet
Flight speed (km.h-1) *
Flight duration (h) *
Flight distance (km) *
2.51 ± 0.04 a
9.96 ± 0.65 a
26.61 ± 1.35 a
2.44 ± 0.05 a
10.91 ± 0.40 a
26.01 ± 1.04 a
2.32 ± 0.05 b
10.82 ± 0.54 a
24.93 ± 1.38 a
Effects of larval diets on flight fuel in the adults
Differences in total amounts of sugar and lipid (mg/g)in the moths from the larvae on diet A, B and C, and their variations before and after flight
Larvae fed diet
68.3 ± 4.5 b
46.6 ± 3.4 b
21.8 ± 1.5 a
405.8 ± 30.5 b
231.4 ± 26.8 a
174.4 ± 14.4 b
65.8 ± 4.9 ab
41.8 ± 2.4 b
24.0 ± 2.9 a
478.6 ± 15.6 a
255.7 ± 16.4 a
222.9 ± 12.1 a
78.0 ± 3.8 a
52.1 ± 4.0 a
26.2 ± 1.8 a
430.8 ± 29.8 ab
240.6 ± 18.6 a
190.3 ± 11.3 a
After more than 10 h's flight, the sugar and lipid levels reduced. The sugar amounts in the moths from diet A, B and C decreased by 31.9%, 36.5% and 33.6%, respectively. Meanwhile, the lipid quantities of the three test groups from diet A, B and C decreased by 43%, 46.6% and 44.2% (Table 5).
Effects of larval diets on larvae cannibalism
Effects of larval diets on sodium intake by the adults
The sodium intake of the one-day-old moths was determined by comparing the mean body weight before and after 10 h of exposure to 3% sodium chloride solution. The average weight increase for 10 males from diet A, B and C was -2.7% ± 0.2%, 3.1% ± 0.6% and 10.7% ± 0.3%, respectively. The latter was significantly higher than the former two. Meanwhile, the weight increase for 10 females from diet A, B and C was -0.04% ± 0.1%, 10.8% ± 0.3% and 10.1% ± 0.4%, respectively. The latter two were significantly higher than the former (F(2,27) = 3.68, P = 0.039).
Exploring the influence of minerals in artificial diets has proven difficult because of impurities in crude natural materials such as wheat germ, soybean flour, yeast and agar, in chemical additives such as amino acids, sterols, vitamins, and fatty acids, and in antioxidants and antimicrobials . To circumvent this difficulty, here we prepared three larval diets and measured their actual sodium and potassium concentrations. Diet A is a conventional diet. Since it has been applied successfully to culture H. armigera in our laboratory for more than twenty years, we assume that sodium content in diet A is suitable for H. armigera larvae. Diet B or C is modified from diet A by replacing a half or whole amount of sodium in Wesson salt mixture  with equal mole of potassium. The sodium concentration in diet B and C decreased by 30.2% and 67.4%, respectively, whereas the potassium concentration increased 11.2% and 21.2%, respectively. Since H. armigera is a phytophagous species and so presumably adapted to ingesting much higher proportion of potassium than characterize conventional mammalian salt mixtures, such as Wesson salt mixture , 10% potassium increase in diet B and 20% increase in diet C should have little effect on H. armigera larvae.
Our results showed H. armigera larvae on diet C grew larger, had a shorter (but not much) development period than those on diet A and B. The larvae previously fed from 1st to 3rd instar on diet C consumed more subsequent diet when they were transferred to diet A or C at 4th instar, comparing to those previously fed on diet A. Moreover, any 4th-instar larvae on diet C consumed a greater amount of food than those on diet A, no matter which diet the larvae had previously ingested from 1st to 3rd instar. These results suggest that the caterpillars on sodium deficient diet practice a compensatory feeding response. Similar results have been reported in Locusta migratoria .
Moreover, compensatory feedings for sodium were also showed in other lepidopterans, especially in males. Male larvae of Thymelicus lineola, for example, consume about 85% of the foliage consumed by female larvae, yet on adult emergence males have a dry weight only 48% of that of the females. Consequently, at emergence males contain 82% of the total body sodium of females and the concentration is twice that in females . This suggests that caterpillars, especially male caterpillars, exhibit compensatory feeding for sodium. Similarly, each notodontid moth Gluphisia septentrionis male contains about 2.3 μg of sodium and had an average body weight of 70 mg, the average sodium concentration in male was 32.9 μg/g fresh weight and approximately 329 μg/g dry weight, 110 times more than that in their primary larval host plant quaking aspen (Populus tremuloides) . Heliothis virescens male larvae were found to have assimilated significantly more sodium from artificial diet than female larvae . In some butterfly species such as Kallimoides rumia, Bicyclus graueri, Euphaedra medon and E. alacris, puddling is uncommon in both sexes, but males do transfer sodium to the female during copulation . A great proportion of sodium in males should then be derived from larval feeding.
Sodium stress may also be tempered by feeding at higher trophic levels . Mormon crickets, Anabrus simplex, showed a higher incidence of cannibalism within migratory bands, and NaCl prefeeding substantially reduced the incidence of cannibalism in laboratory test . Cannibalism was also observed in H. armigera larvae, especially under poorer nutritional conditions or greater larval rearing density . Is cannibalism a strategy for H. armigera larvae to obtain sodium? Our results showed that the larval survival rate from 1st to 3rd instar of the three test groups fed on diet A, B and C was 85.6 ± 1.7%, 70.4 ± 2.1%, and 65.6 ± 1.9% respectively, the former was significantly higher than the latter two. In cannibalism test, when a 5th-instar victim from diet A and a 5th-instar cannibal from diet A, B or C were left together for 1 hour, many more cannibals from diet C ate their victims. Furthermore, when a 5th-instar victim from diet A, B or C was provided, a 5th-instar cannibal preferred victims with putatively higher sodium concentrations. Our results demonstrated that H. armigera larvae frequently cannibalized others under sodium deficient condition.
Our results also showed that the moths from diet A obtained less average weight increase than those from B or C after having had the opportunity to feed on 3% sodium chloride solution for 10 h scotophase period. This demonstrated that sodium-deprived H. armigera adults could resort to puddling to compensate for the shortage of sodium. If considing the excrement, the differences among the resulting moths were much greater, since we noted the bottoms of the jars containing diet B or C originated moths were wetter than those containing diet A originated moths.
In present paper, we tested the flight capabilities of the moths from diet A, B and C. We found that the moths from diet A and B flew more rapidly and covered slightly longer distances than those from diet C, with similar sugar and lipid utilization rates among the three test groups. These findings may have two possible explanations, of which the deduction proposed by Downes  and Arms et al.  is one. They suggested that sodium may be important for maintaining high neuromuscular activity and may affect flight speed. The moths originating from sodium-deficient diets had putatively lower sodium concentrations in their body and flew slowly. The other explanation is size differences among the moths from three different diets. Our results showed that the moths from diet B and C had lighter body weights and consequently smaller body size, and tend to fly slower.
Larvae on diet C consume a large quantity of food, and grew larger than caterpillars on diet A. However, the resulting pupae and adults from diet C have lighter body weights than those from diet A. Moreover, the adults from diet C did not have the highest body lipid content. The profound discrepancy demonstrated that sodium-deficient individuals discarded larger quantities of waste products such as exuviae and meconium during metamorphosis. Further research will shed light on this issue.
In present paper, we report a comparative analysis of the effects of dietary sodium on performance, flight and compensation strategies in H. armigera, a rarely mud-puddling insect species in nature. We found that sodium deficiency resulted in rapid growth and development of larvae, decreased larvae survival rate, and reduced flight speed of adults. H. armigera ingested a large quantity of larval food, increased larval cannibalism incidence and harvested sodium at adult stage to compensate for sodium deficiency.
The research is supported by National Natural Sciences Foundation of China (Project No. 30671368) and Scientific Research Program of the Higher Education Institution of XinJiang (FSRPHEXJ) (No. XJEDU2006123). We thank Drs Z. Han, F. Li and S. Dong of our laboratory for useful discussions during the course of this research, as well as Dr X. Liu for helping with tethered flight tests. We offer our sincere thanks to Dr Juergen Heinze, Dr David Raubenheimer and several anonymous reviewers for many corrections and helpful suggestions on the earlier version of the manuscript.
- Arms K, Feeny P, Lederhouse R: Sodium: stimulus for puddling behavior by tiger swallowtail butterflies, Papilio glaucus. Science. 1974, 185: 372-374. 10.1126/science.185.4148.372.View ArticlePubMedGoogle Scholar
- Zeiske W: Insect ion homeostasis. J Exp Biol. 1992, 172: 323-334.PubMedGoogle Scholar
- Kaspari M, Yanoviak S, Dudley R: On the biogeography of salt limitation: A study of ant communities. Proc Natl Acad Sci USA. 2008, 105: 17848-17851. 10.1073/pnas.0804528105.PubMed CentralView ArticlePubMedGoogle Scholar
- Shen K, Wang H, Shao L, Xiao K, Shu J, Xu T, Li G: Mud-puddling in the yellow-spined bamboo locust, Ceracris kiangsu (Oedipodidae: Orthoptera): Does it detect and prefer salts or nitrogenous compounds from human urine?. J Insect Physiol. 2008, 55: 78-84. 10.1016/j.jinsphys.2008.10.011.View ArticlePubMedGoogle Scholar
- Trumper S, Simpson S: Regulation of salt intake by nymphs of Locusta migratoria. J Insect Physiol. 1993, 39: 857-864. 10.1016/0022-1910(93)90118-B.View ArticleGoogle Scholar
- Trumper S, Simpson S: Mechanisms regulating salt intake in fifth-instar nymphs of Locusta migratoria. Physiol Entomol. 1994, 19: 203-215. 10.1111/j.1365-3032.1994.tb01044.x.View ArticleGoogle Scholar
- Meneely G, Battarbee H: Sodium and potassium. Nutr Rev. 1976, 34: 225-235.View ArticlePubMedGoogle Scholar
- Jachmann H, Zweypfenning R, Molen Van der J: Effects of haemolymph free cations on blowfly taste receptor responses. J Insect Physiol. 1982, 28: 943-946. 10.1016/0022-1910(82)90110-X.View ArticleGoogle Scholar
- Blair-West J, Coghlan J, Denton D, Nelson J, Orchard E, Scoggins B, Wright R, Myers K, Junqueira C: Physiological, morphological and behavioural adaptation to a sodium deficient environment by wild native Australian and introduced species of animals. Nature. 1968, 217: 922-928. 10.1038/217922a0.View ArticlePubMedGoogle Scholar
- Rode K, Chapman C, Chapman L, McDowell L: Mineral resource availability and consumption by colobus in Kibale National Park, Uganda. Int J Primatol. 2003, 24: 541-573. 10.1023/A:1023788330155.View ArticleGoogle Scholar
- Liu X, Yu J, Li M, Li G, Yang Z, Huang X, Hu D: Preliminary study on the nutrient character of the stable food bamboo of captive giant panda. Acat Theriol Sin. 2001, 21: 314-317.Google Scholar
- Smedley S, Eisner T: Sodium uptake by puddling in a moth. Science. 1995, 270: 1816-1818. 10.1126/science.270.5243.1816.View ArticlePubMedGoogle Scholar
- Chapman R: The insects: structure and function. 1998, Cambridge Univ PressView ArticleGoogle Scholar
- Botkin D, Jordan P, Dominski A, Lowendorf H, Hutchinson G: Sodium dynamics in a northern ecosystem. Proc Natl Acad Sci USA. 1973, 70: 2745-2748. 10.1073/pnas.70.10.2745.PubMed CentralView ArticlePubMedGoogle Scholar
- Belovsky G, Jordan P: Sodium dynamics and adaptations of a moose population. J Mammal. 1981, 613-621. 10.2307/1380408.Google Scholar
- Simpson S, Sword G, Lorch P, Couzin I: Cannibal crickets on a forced march for protein and salt. Proc Natl Acad Sci USA. 2006, 103: 4152-4156. 10.1073/pnas.0508915103.PubMed CentralView ArticlePubMedGoogle Scholar
- Emmons L, Stark N: Elemental composition of a natural mineral lick in Amazonia. Biotropica. 1979, 311-313. 10.2307/2387925.Google Scholar
- Tracy B, McNaughton S: Elemental analysis of mineral lick soils from the Serengeti National Park, the Konza Prairie and Yellowstone National Park. Ecography. 1995, 91-94. 10.1111/j.1600-0587.1995.tb00122.x.Google Scholar
- Rothman J, Van Soest P, Pell A: Decaying wood is a sodium source for mountain gorillas. Biol Let. 2006, 2: 321-324. 10.1098/rsbl.2006.0480.View ArticleGoogle Scholar
- Reinthal W: About the" pumping action" of a Papilio at water. J Lepid Soc. 1963, 17: 35-36.Google Scholar
- Downes J: Lepidoptera feeding at puddle-margins, dung, and carrion. J Lepid Soc. 1973, 27: 89-99.Google Scholar
- Berger T, Lederhouse R: Puddling by single male and female tiger swallowtails, Papilio glaucus L.(Papilionidae). J Lepid Soc. 1985, 39: 339-340.Google Scholar
- Sculley C, Boggs C: Mating systems and sexual division of foraging effort affect puddling behaviour by butterflies. Ecol Entomol. 1996, 21: 193-197. 10.1111/j.1365-2311.1996.tb01187.x.View ArticleGoogle Scholar
- Beck J, Muèhlenberg E, Fiedler K: Mud-puddling behavior in tropical butterflies: in search of proteins or minerals?. Oecologia. 1999, 119: 140-148. 10.1007/s004420050770.View ArticleGoogle Scholar
- Hall J, Willmott K: Patterns of feeding behaviour in adult male riodinid butterflies and their relationship to morphology and ecology. Biol J Linn Soc. 2000, 69: 1-23. 10.1111/j.1095-8312.2000.tb01666.x.View ArticleGoogle Scholar
- Burger J, Gochfeld M: Smooth-billed ani (Crotophaga ani) predation on butterflies in Mato Grosso, Brazil: risk decreases with increased group size. Behav Ecol Sociobiol. 2001, 49: 482-492. 10.1007/s002650100327.View ArticleGoogle Scholar
- Scriber J: A female Papilio canadensis (Lepidoptera: Papilionidae) puddles with males. Am Midland Nat. 2002, 147: 175-178. 10.1674/0003-0031(2002)147[0175:AFPCLP]2.0.CO;2.View ArticleGoogle Scholar
- Boggs C, Dau B: Resource specialization in puddling Lepidoptera. Environ Entomol. 2004, 33: 1020-1024. 10.1603/0046-225X-33.4.1020.View ArticleGoogle Scholar
- Molleman F, Grunsven R, Liefting M, Zwaan B, Brakefield P: Is male puddling behaviour of tropical butterflies targeted at sodium for nuptial gifts or activity?. Biol J Linn Soc. 2005, 86: 345-361. 10.1111/j.1095-8312.2005.00539.x.View ArticleGoogle Scholar
- Gadagkar R: Evolution: A subaltern view of eusociality. Science. 2006, 314: 1391-1392. 10.1126/science.1135094.View ArticleGoogle Scholar
- Payne J, King E: Lepidoptera associated with pig carrion. Lepidop Soc. 1969, 23: 191-195.Google Scholar
- Adler P: Soil- and puddle-visiting habits of moths. J Lepid Soc. 1982, 36: 161-173.Google Scholar
- Banziger H: Moths with a taste for tears: Insect that live off the tears of mammals find the secretions of tasty and nutritious food. The moths wisely favour mild-mannered herbivores as their victims. New Sci. 1990, 48-51.Google Scholar
- Butler C: The choice of drinking water by the honeybee. J Exp Biol. 1940, 17: 253-261.Google Scholar
- Adler P: Nocturnal occurrences of leafhoppers (Homoptera: Cicadellidae) at soil. J Kansas Entomol Soc. 1982, 73-74.Google Scholar
- McDowell L: Minerals in animal and human nutrition. 2003, Elsevier Health SciencesGoogle Scholar
- Bell F: Perception of sodium and sodium appetite in farm animals. Sodium in Agriculture. Cantebury, UK. Edited by: Phillips CJC, Chiy PC. 1995, 82-90.Google Scholar
- Faber W, Pehrson Å, Jordan P: Seasonal use of salt blocks by mountain hares in Sweden. J Wildl Manage. 1993, 842-846.Google Scholar
- Fox L, Krausman P, Morrison M, Noon T: Mineral content of Sonoran pronghorn forage. California Fish and Game. 2000, 86: 159-174.Google Scholar
- Krishnamani R, Mahaney W: Geophagy among primates: adaptive significance and ecological consequences. Anim Behav. 2000, 59: 899-915. 10.1006/anbe.1999.1376.View ArticlePubMedGoogle Scholar
- King A: Heliothis/Helicoverpa (Lepidoptera: Noctuidae). 1994, Wallingford: CAB InternationalGoogle Scholar
- Bruce T, Cork A: Electrophysiological and behavioral responses of female Helicoverpa armigera to compounds identified in flowers of African marigold, Tagetes erecta. J Chem Ecol. 2001, 27: 1119-1131. 10.1023/A:1010359811418.View ArticlePubMedGoogle Scholar
- Xu H, Li G, Liu M, Xing G: Oviposition deterrents in larval frass of the cotton boll worm, Helicoverpa armigera (Lepidoptera: Noctuidae): Chemical identification and electroantennography analysis. J Insect Physiol. 2006, 52: 320-326. 10.1016/j.jinsphys.2005.11.011.View ArticlePubMedGoogle Scholar
- Liu M, Yu H, Li G: Oviposition deterrents from eggs of the cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae): Chemical identification and analysis by electroantennogram. J Insect Physiol. 2008, 54: 656-662. 10.1016/j.jinsphys.2008.01.002.View ArticlePubMedGoogle Scholar
- Wesson L: A modification of the osbornemendel salt mixture containing only inorganic constituents. Science. 1932, 75: 339-340. 10.1126/science.75.1943.339.View ArticlePubMedGoogle Scholar
- Naranjo S: Comparative flight behavior of Diabrotica virgifera virgifera and Diabrotica barberi in the laboratory. Entomol Exp Appl. 1990, 55: 79-90. 10.1007/BF00188230.View ArticleGoogle Scholar
- Murata M, Tojo S: Flight capability and fatty acid level in triacylglycerol of long-distance migratory adults of the common cutworm, Spodoptera litura. Zool Sci. 2004, 21: 181-188. 10.2108/zsj.21.181.View ArticlePubMedGoogle Scholar
- Folch J: Lees M., and Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 226: 497-509.
- Ayali A, Pener M: Density-dependent phase polymorphism affects response to adipokinetic hormone in Locusta. Comp Biochem Physiol. 1992, 101A: 549-552. 10.1016/0300-9629(92)90507-M.View ArticleGoogle Scholar
- Ziegler R: Hyperglycaemic factor from the corpora cardiaca of Manduca sexta (L.) (Lepidoptera: Sphingidae). Gen Comp Endocrinol. 1979, 39: 350-357. 10.1016/0016-6480(79)90132-1.View ArticlePubMedGoogle Scholar
- Duncan D: Multiple range and multiple F tests. Biometrics. 1955, 1-42. 10.2307/3001478.Google Scholar
- Reinecke J: Nutrition: artificial diets. Comprehensive insect physiology, biochemistry and pharmacology. Edited by: Kerkut GA, Gilbert LI. 1985, Pergamon Press, UK, 4: 391-419.Google Scholar
- Trumper S, Simpson S: Regulation of salt intake by nymphs of Locusta migratoria. J Insect Physiol. 1993, 39: 857-857. 10.1016/0022-1910(93)90118-B.View ArticleGoogle Scholar
- Pivnick K, McNeil J: Puddling in butterflies: sodium affects reproductive success in Thymelicus lineola. Physiol Entomol. 1987, 12: 461-472. 10.1111/j.1365-3032.1987.tb00773.x.View ArticleGoogle Scholar
- Popham H, Shelby K: Uptake of dietary micronutrients from artificial diets by larval Heliothis virescens. J Insect Physiol. 2006, 52: 771-777. 10.1016/j.jinsphys.2006.04.005.View ArticlePubMedGoogle Scholar
- Kakimoto T, Fujisaki K, Miyatake T: Egg laying preference, larval dispersion, and cannibalism in Helicoverpa armigera (Lepidoptera: Noctuidae). Ann Entomol Soc Am. 2003, 96: 793-798. 10.1603/0013-8746(2003)096[0793:ELPLDA]2.0.CO;2.View ArticleGoogle Scholar
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