Outlining eicosanoid biosynthesis in the crustacean Daphnia
© Heckmann et al; licensee BioMed Central Ltd. 2008
Received: 28 March 2008
Accepted: 14 July 2008
Published: 14 July 2008
Eicosanoids are biologically active, oxygenated metabolites of three C20 polyunsaturated fatty acids. They act as signalling molecules within the autocrine or paracrine system in both vertebrates and invertebrates mainly functioning as important mediators in reproduction, the immune system and ion transport. The biosynthesis of eicosanoids has been intensively studied in mammals and it is known that they are synthesised from the fatty acid, arachidonic acid, through either the cyclooxygenase (COX) pathway; the lipoxygenase (LOX) pathway; or the cytochrome P450 epoxygenase pathway. However, little is still known about the synthesis and structure of the pathway in invertebrates.
Here, we show transcriptomic evidence from Daphnia magna (Crustacea: Branchiopoda) together with a bioinformatic analysis of the D. pulex genome providing insight on the role of eicosanoids in these crustaceans as well as outlining a putative pathway of eicosanoid biosynthesis. Daphnia appear only to have one copy of the gene encoding the key enzyme COX, and phylogenetic analysis reveals that the predicted protein sequence of Daphnia COX clusters with other invertebrates. There is no current evidence of an epoxygenase pathway in Daphnia; however, LOX products are most certainly synthesised in daphnids.
We have outlined the structure of eicosanoid biosynthesis in Daphnia, a key genus in freshwater ecosystems. Improved knowledge of the function and synthesis of eicosanoids in Daphnia and other invertebrates could have important implications for several areas within ecology. This provisional overview of daphnid eicosanoid biosynthesis provides a guide on where to focus future research activities in this area.
The COX enzyme exists in at least two isoforms in mammals, COX-1 and COX-2, which are inhibited by conventional non-steroidal anti-inflammatory drugs (NSAID) such as ibuprofen and aspirin, but only one COX isoform is generally present in invertebrates and lower vertebrates . Mammalian COX-1 catalyses the generation of prostaglandins (PG) involved in many basic physiological functions such as regulation of blood pressure, gastric mucosal protection, maintenance of homeostasis, and reproductive and nervous system function; while PGs metabolised by COX-2 are involved in inflammation, ovulation and mitogenesis [3, 4]. COX-1 is a constitutive enzyme (i.e. it is constantly expressed) found in the cell membranes of most mammalian tissues, while COX-2 is an inducible enzyme, not found in all cell types, that is located in nuclear membranes . In the mammalian LOX pathway AA is converted by different LOXs into hydroperoxyeicosatetraenoic acids (HPETE) that may be further metabolised into hydroxyeicosatetraenoic acids (HETE) by glutathione peroxidase. Leukotrienes are synthesised from 5-HPETE by 5-LOX, while 8-LOX, 12-LOX and 15-LOX catalyse the production of different lipoxins from 8-HPETE, 12-HPETE and 15-HPETE (Fig. 1).
Research on eicosanoids has mainly been mammalian-driven and has lately been aimed at designing NSAIDs that are COX-2 selective due to the potential negative side-effects of COX-1 inhibition, which may affect the gastrointestinal tract, heart and kidneys . Eicosanoids act at both the extracellular and intracellular level by interacting with distinct transmembrane G-protein coupled receptors (extra- and intracellular) and nuclear peroxisomal proliferator-activated receptors (PPAR) . Activated G-proteins may, depending on cell type, stimulate second messengers such as cyclic adenosine monophosphate (cAMP) and/or intracellular calcium release . PPAR are transcription factors which also have a role in ligand binding (eicosanoids), so directly influencing the expression of target genes involved in e.g. controlling prenatal and postnatal development . These examples emphasise the biological significance of eicosanoids.
Less is known about eicosanoids in non-mammalian species; however, during the last three decades considerable evidence has been gathered concerning their synthesis and action. Eicosanoids have now been identified in almost every major metazoan phyla including some plants . There is general consensus that eicosanoids act as autocrine or paracrine signallers (also referred to as local hormones) in both vertebrates and invertebrates, where they mainly function as important mediators in reproduction, the immune system and ion transport . It is clear from a number of reports that eicosanoid generation is subject to inhibition by NSAIDs in a wide range of invertebrates [1, 2]. Moreover, a COX derived mechanism similar to the mammalian biosynthesis of PGs has been proposed in the coral Plexaura homomalla . There is also evidence of a LOX derived pathway being present in invertebrates based on the work of Ragab and colleagues  on the primitive wingless insect, the firebrat Thermobia domestica; although little is known about the structure of the pathway. Overall, invertebrate eicosanoid biosynthesis seems to have a simpler structure than its mammalian counterpart, as seen with the COX pathway , but also appears to be split into two instead of three pathways. There is currently no proof of an epoxygenase pathway being present in invertebrates .
There is little doubt that disruption of eicosanoid biosynthesis may upset many important physiological functions in both invertebrates and vertebrates, which can have serious consequences for both the individual and the population. Current evidence suggests that the main mode of action of the NSAID ibuprofen in D. magna relates to interruption of eicosanoid biosynthesis which reduces fecundity [9–12]. Eicosanoids may therefore play a pivotal role in daphnid reproduction. A wealth of synthetic and natural chemicals may affect invertebrate reproduction through endocrine disruption with one of the best known examples being imposex (masculinisation) of female molluscs caused by exposure to tributyl tin (TBT) . It is therefore important to understand eicosanoid biosynthesis in Daphnia, and invertebrates in general, to fully recognize the potential mode of action of endocrine disrupters and how they may affect natural invertebrate populations.
Recently, the genome of D. pulex was fully sequenced , and action within the Daphnia Genomics Consortium has already been taken to start sequencing the D. magna genome [15, 16]. In the meantime genes identified in D. pulex serve as a model for understanding eicosanoid biosynthesis, control and disruption in Daphnia. Here we present an overview of the putative eicosanoid biosynthesis in Daphnia based on annotation of genes from the D. pulex genome supported by recently published transcriptomic data (real-time quantitative PCR) of ibuprofen-stressed D. magna .
Putative genes related to eicosanoid biosynthesis were identified on the D. pulex genome website  through using several bioinformatic search tools such as GO (Gene Ontology) , KEGG (Kyoto Encyclopedia of Genes and Genomes)  and matches against InterPro protein domains. Annotation of these genes were verified through BLAST searches performed against Swissprot protein and non-redundant protein sequence databases via the D. pulex genome website .
Invertebrate and vertebrate COX protein sequences were retrieved from GenBank  and Ensembl  for phylogenetic analysis. Since no arthropod COX protein sequences were available that could aid the phylogeny with respect to D. pulex, we searched the GenBank Expressed Sequence Tag (EST) division (est_others) using the BLAST algorithm (tblastn) to obtain additional arthropod COX sequences. Sequences that significantly resembled the sea squirt Ciona intestinalis COX amino acid sequence (E-value < 1e-20) were retrieved and included in the dataset. Six ESTs were obtained, representing two malacostracan species (Crustacea): Four ESTs were derived from Homarus americanus (accession numbers DV772953, DV774102, EH401871 and FD699680) and two were derived from Petrolisthes cinctipes (accession numbers FE773225, FE820815). After translating the nucleotide sequences to putative amino acids, it appeared that one H. americanus sequence (FD699680) did not overlap with the other three sequences. The three remaining sequences constituted two slightly deviating amino acid sequences; DV774102 differed from EH401871 and DV772953. The four H. americanus sequences were combined to one sequence with variable and missing positions specified as 'unknown'. Furthermore, the inferred amino acid sequences of the two P. cinctipes ESTs were similar, and P. cinctipes was thus included once in the dataset. Note that these additional crustacean entries were EST derived, single pass sequenced, and therefore not guaranteed to be free from sequencing errors.
The sequence COX dataset was aligned using ClustalW2 , manually edited after inspection and subsequently analysed using the Gblocks web-server  to pinpoint and remove unreliably aligned regions. This analysis allowed for gaps in the final alignment. ModelGenerator  was applied to obtain a model of sequence evolution (gamma distribution with four rate categories) using the Akaike Information Criterion. The predicted model, WAG+I+G, was specified in Phyml v2.4.4  and a dendrogram was constructed using Maximum Likelihood (BIONJ  starting tree). Phylogenetic analysis used 100 bootstrap replicates. Finally, the obtained topology was visualised using TreeView .
Results and Discussion
Putative Daphnia pulex genes associated with eicosanoid biosynthesis
Prostaglandin D2 synthase A
Prostaglandin D2 synthase B
Prostaglandin E2 synthase
Carbonyl reductase 1 (PG 9-ketoreductase)
Leukotriene A4 hydrolase
Leukotriene B4 12-hydroxydehydrogenase
Prostanoid receptor EP4 isoform A
Prostanoid receptor EP4 isoform B
Like the putative Daphnia COX pathway, the structure of the daphnid LOX pathway also appeared to be more simple than its mammalian counterpart, which confirms previous findings . There was gene sequence evidence that indicated the presence of three leukotrienes (LT) in Daphnia: LTA4, LTB4 and 12-oxo LTB4 (Table 1). This is, however, doubtful since no LTs, nor the precursor 5-HPETE, have been identified in invertebrates and lower vertebrates to date . It is possible that the putative LTA4 hydrolase (LTA4H), which is a bi-functional enzyme in mammals, having both LTA4 hydrolase and aminopeptidase activity, only has aminopeptidase activity in daphnids as has been reported in Caenorhabditis elegans . However, recent evidence shows that bi-functionality of LTA4H is only six point mutations away in yeast compared to the mammalian enzyme . There is reason to believe that daphnid LTA4H may be bi-functional, and thus able to convert LTA4 into LTB4; a hypothesis that we will test in future experiments. Furthermore, transcriptomic evidence from D. magna shows that the expression of leukotriene B4 12-hydroxydehydrogenase (Ltb4dh) increases with increasing concentration of the eicosanoid-inhibiting drug ibuprofen . This does not prove that LTs are present in daphnids, but yet again we cannot rule out the possibility. The enzyme (LTB4DH) encoded by Ltb4dh is also bi-functional in mammals regulating eicosanoids by rapidly degrading different E and F series PGs and LTB4  (see below). The former function of LTB4DH (PG catabolism) appears to be the most likely in daphnids until solid proof exits about the presence of LTs (Fig. 2).
The presence of lipoxins is, however, more likely, and bioinformatic information from D. pulex suggests that at least two LOX enzymes are present (Table 1). LOX enzymes have been found in all organisms studied, from bacteria to man. The two putative D. pulex LOXs are both composed by two domains; an N-terminal lipase domain belonging to the InterPro protein family 734 (IPR000734) and a C-terminal LOX LH2 domain (IPR001024). This resembles mammalian LOX enzymes that are also comprised of two domains; a regulatory N-terminal domain that is similar to mammalian lipases and a catalytic LOX domain (C-terminal) . LOX LH2 is the only LOX related domain that has been identified in the D. pulex genome. Other known LOX domains include e.g. mammalian LOX (IPR001885) and LOX, C-terminal (IPR013819). There are 13 D. pulex gene models that contain the LOX LH2 domain , but only two genes contain both an N-terminal domain similar to mammalian lipases (IPR000724) and a C-terminal LOX domain (Table 1). Further investigations are needed to specify what type of LOX enzyme these two Daphnia sequences represent prior to analysing their phylogenetic relationship. However, it is likely that they could be 8-LOX and 12-LOX, which synthesise different lipoxins from 8-HPETE and 12-HPETE, as these LOXs have been identified in a range of invertebrate species [8, 33, 34].
Two enzymes, 15-hydroxyprostaglandin dehydrogenase (PGDH) and LTB4DH are known to irreversibly inactivate bioactive eicosanoids in mammals. Both enzymes are key in regulating the hormonal-like action of eicosanoids by rapidly degrading PGE2, PGF2α, and LTB4, as overproduction of these potent mediators may have serious physiological effects such as initiating inflammation . It appeared that LTB4DH fulfils this regulatory function single-handedly in daphnids, as there was no indication of PGDH being present (Table 1, Fig. 2).
The bioinformatic and transcriptomic evidence from D. pulex and D. magna (Fig. 2) suggests that PGs (e.g. PGH2, PGE2 and PGF2α), lipoxins and possibly LTs could be present in daphnids. Low similarity of TXA2 synthase and PGD2 synthase to ortholog proteins from other genomes (Fig. 2) render the presence of PGD2 and TXA2 to be less certain in daphnids, although this could merely be due to daphnids having more divergent versions of these proteins. Nevertheless, it seems most likely that daphnids do not produce TXA2 since the gene encoding TXA2 synthase has not been identified in the genome of C. intestinalis . The LOX encoding genes identified in D. pulex were also slightly doubtful due to the same reasons, but it would be more probable that these enzymes are present in Daphnia as both 8-LOX and 12-LOX derived lipoxins are common in invertebrates [8, 33, 34]. PGA2 may also be present in daphnids as it is non-enzymatically rearranged from PGE2 and has been detected in several arthropods ; but until verified by mass spectrometry or the like it remains speculative what eicosanoids are present in Daphnia. Moreover, the annotation of genes from the daphnid eicosanoid biosynthesis (and other daphnid genes for that matter) should improve as more invertebrate genomes become sequenced and annotated.
The possible roles of eicosanoids in daphnids have already been suggested from several invertebrate studies, including D. magna , where both prostanoids and lipoxygenase products appear to be important agents in oogenesis (especially vitellogenesis) and embryogenesis [35–38]. For instance, PGE2 is known to initiate egg-laying behaviour in several insect species (e.g. orthopterans), where it seems to regulate muscle contractions in the ovarian musculature . Furthermore, many of the above-mentioned eicosanoids have likewise been identified as important mediators in arthropod immune systems (both COX and LOX products)  and ion transport physiology (mainly PGE2 and PGF2α) . Until more integrated phenotypic and genomic evidence exists it is difficult to infer an exact role for eicosanoids in daphnids, as they may be involved in several processes and act in different tissues. Nevertheless, it is almost certain that eicosanoids play vital roles in the functioning of processes key to daphnid reproduction and survival [9, 10, 12]. Finally, bioinformatic evidence from the D. pulex genome also revealed that two prostanoid G-protein coupled receptors may be present, thus further supporting the evidence that eicosanoids are bioactive agents in daphnids (Table 1).
Eicosanoids are key molecules, involved in the function of fundamentally important biological systems. A better understanding of their biochemistry and genetic control in invertebrates will help to improve our understanding of their significance in these organisms. Here we have outlined a putative structure of eicosanoid biosynthesis in Daphnia, a key macroinvertebrate in freshwater ecosystems. It would seem, from transcriptomic and phenotypic evidence, that eicosanoids play a pivotal role in daphnid reproduction ; but their importance in other physiological functions such as the immune system remains to be investigated. Improved knowledge of the function and synthesis of eicosanoids in Daphnia and other invertebrates could have very important implications for several areas within ecology including ecological risk assessment. This provisional overview of daphnid eicosanoid biosynthesis provides a guide on where to focus future research activities in this area.
We gratefully acknowledge the financial support of AstraZeneca, Syngenta, NERC (project NER/D/S/2002/00413 "The population and molecular stress responses of an ecotoxicology indicator species") and The Research Endowment Trust Fund of the University of Reading. We thank the Daphnia Genomics Consortium (DGC) for giving us the opportunity of annotating D. pulex genes related to eicosanoid biosynthesis, and Vibeke Simonsen (University of Aarhus, National Environmental Research Institute) for critically reading the manuscript. The sequencing and portions of the analyses were performed at the DOE Joint Genome Institute under the auspices of the U.S. Department of Energy's Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Livermore National Laboratory under Contract No. W-7405-Eng-48, Lawrence Berkeley National Laboratory under Contract No. DE-AC02-05CH11231, Los Alamos National Laboratory under Contract No. W-7405-ENG-36 and in collaboration with the DGC . Additional analyses were performed by wFleaBase , developed at the Genome Informatics Lab of Indiana University with support to Don Gilbert from the National Science Foundation and the National Institutes of Health. Coordination infrastructure for the DGC is provided by The Center for Genomics and Bioinformatics at Indiana University, which is supported in part by the METACyt Initiative of Indiana University, funded in part through a major grant from the Lilly Endowment, Inc. Our work benefits from, and contributes to the DGC .
- Stanley DW: Eicosanoids in invertebrate signal transduction systems. 2000, New Jersey , Princeton University PressGoogle Scholar
- Rowley AF, Vogan CL, Taylor GW, Clare AS: Prostaglandins in non-insectan invertebrates: recent insights and unsolved problems. Journal of Experimental Biology. 2005, 208 (1): 3-14. 10.1242/jeb.01275.View ArticlePubMedGoogle Scholar
- McGettigan P, Henry D: Current problems with non-specific COX inhibitors. Current Pharmaceutical Design. 2000, 6 (17): 1693-1724. 10.2174/1381612003398690.View ArticlePubMedGoogle Scholar
- Ye XQ, Hama K, Contos JJA, Anliker B, Inoue A, Skinner MK, Suzuki H, Amano T, Kennedy G, Arai H, Aoki J, Chun J: LPA(3)-mediated lysophosphatidic acid signalling in embryo implantation and spacing. Nature. 2005, 435 (7038): 104-108. 10.1038/nature03505.PubMed CentralView ArticlePubMedGoogle Scholar
- Funk CD: Prostaglandins and leukotrienes: Advances in eicosanoid biology. Science. 2001, 294 (5548): 1871-1875. 10.1126/science.294.5548.1871.View ArticlePubMedGoogle Scholar
- Feige JN, Gelman L, Michalik L, Desvergne B, Wahli W: From molecular action to physiological outputs: Peroxisome proliferator-activated receptors are nuclear receptors at the crossroads of key cellular functions. Progress in Lipid Research. 2006, 45 (2): 120-159. 10.1016/j.plipres.2005.12.002.View ArticlePubMedGoogle Scholar
- Valmsen K, Jarving I, Boeglin WE, Varvas K, Koljak R, Pehk T, Brash AR, Samel N: The origin of 15R-prostaglandins in the Caribbean coral Plexaura homomalla: Molecular cloning and expression of a novel cyclooxygenase. Proceedings of the National Academy of Sciences of the United States of America. 2001, 98 (14): 7700-7705. 10.1073/pnas.131022398.PubMed CentralView ArticlePubMedGoogle Scholar
- Ragab A, Durand J, Bitsch C, Chap H, Rigaud M: The lipoxygenase pathway of arachidonic acid metabolism in reproductive tissues of the firebrat, Thermobia domestica (Thysanura). Insect Biochemistry. 1991, 21 (3): 321-326. 10.1016/0020-1790(91)90022-7.View ArticleGoogle Scholar
- Hayashi Y, Heckmann LH, Callaghan A, Sibly RM: Reproduction recovery of the crustacean Daphnia magna following chronic exposure to ibuprofen. Ecotoxicology. 2008, 17: 246-251. 10.1007/s10646-008-0191-3.View ArticlePubMedGoogle Scholar
- Heckmann LH, Callaghan A, Hooper HL, Connon R, Hutchinson TH, Maund SJ, Sibly RM: Chronic toxicity of ibuprofen to Daphnia magna: Effects on life history traits and population dynamics. Toxicology Letters. 2007, 172 (3): 137-145. 10.1016/j.toxlet.2007.06.001.View ArticlePubMedGoogle Scholar
- Heckmann LH, Connon R, Hutchinson TH, Maund SJ, Sibly RM, Callaghan A: Expression of target and reference genes in Daphnia magna exposed to ibuprofen. BMC Genomics. 2006, 7: 175-10.1186/1471-2164-7-175.PubMed CentralView ArticlePubMedGoogle Scholar
- Heckmann LH, Sibly RM, Connon R, Hooper HL, Hutchinson TH, Maund SJ, Hill CJ, Bouetard A, Callaghan A: Systems biology meets stress ecology: Linking molecular and organismal stress responses in Daphnia magna. Genome Biology. 2008, 9 (2): R40-10.1186/gb-2008-9-2-r40.PubMed CentralView ArticlePubMedGoogle Scholar
- Hutchinson TH: Reproductive and developmental effects of endocrine disrupters in invertebrates: in vitro and in vivo approaches. Toxicology Letters. 2002, 131 (1-2): 75-81. 10.1016/S0378-4274(02)00046-2.View ArticlePubMedGoogle Scholar
- JGI Genome Portal. [http://www.jgi.doe.gov/Daphnia]
- wFleaBase. [http://wFleaBase.org]
- Daphnia Genomics Consortium. [http://daphnia.cgb.indiana.edu]
- Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, Harris MA, Hill DP, Issel-Tarver L, Kasarskis A, Lewis S, Matese JC, Richardson JE, Ringwald M, Rubin GM, Sherlock G: Gene Ontology: tool for the unification of biology. Nature Genetics. 2000, 25 (1): 25-29. 10.1038/75556.PubMed CentralView ArticlePubMedGoogle Scholar
- Kanehisa M, Goto S: KEGG: Kyoto Encyclopedia of Genes and Genomes. Nucleic Acids Research. 2000, 28 (1): 27-30. 10.1093/nar/28.1.27.PubMed CentralView ArticlePubMedGoogle Scholar
- GenBank. [http://www.ncbi.nlm.nih.gov/Genbank/]
- Ensembl. [http://www.ensembl.org/]
- Chenna R, Sugawara H, Koike T, Lopez R, Gibson TJ, Higgins DG, Thompson JD: Multiple sequence alignment with the Clustal series of programs. Nucleic Acids Research. 2003, 31 (13): 3497-3500. 10.1093/nar/gkg500.PubMed CentralView ArticlePubMedGoogle Scholar
- Castresana J: Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000, 17 (4): 540-552.View ArticlePubMedGoogle Scholar
- Keane TM, Creevey CJ, Pentony MM, Naughton TJ, McLnerney JO: Assessment of methods for amino acid matrix selection and their use on empirical data shows that ad hoc assumptions for choice of matrix are not justified. BMC Evolutionary Biology. 2006, 6: 29-10.1186/1471-2148-6-29.PubMed CentralView ArticlePubMedGoogle Scholar
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology. 2003, 52 (5): 696-704. 10.1080/10635150390235520.View ArticlePubMedGoogle Scholar
- Gascuel O: BIONJ: An improved version of the NJ algorithm based on a simple model of sequence data. Molecular Biology and Evolution. 1997, 14: 685-695.View ArticlePubMedGoogle Scholar
- Page RDM: TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci. 1996, 12 (4): 357-358.PubMedGoogle Scholar
- Järving R, Järving I, Kurg R, Brash AR, Samel N: On the evolutionary origin of cyclooxygenase (COX) isozymes - Characterization of marine invertebrate COX genes points to independent duplication events in vertebrate and invertebrate lineages. Journal of Biological Chemistry. 2004, 279 (14): 13624-13633. 10.1074/jbc.M313258200.View ArticlePubMedGoogle Scholar
- Morgan EL, Maskrey BH, Rowley AF: At what stage in metazoan evolution did leukotriene generation first appear? - key insights from cartilaginous fish. Developmental and Comparative Immunology. 2005, 29 (1): 53-59. 10.1016/j.dci.2004.05.001.View ArticlePubMedGoogle Scholar
- Baset HA, Ford-Hutchinson AW, O'Neill GP: Molecular cloning and functional expression of a Caenorhabditis elegans aminopeptidase structurally related to mammalian leukotriene A(4) hydrolases. Journal of Biological Chemistry. 1998, 273 (43): 27978-27987. 10.1074/jbc.273.43.27978.View ArticlePubMedGoogle Scholar
- Tholander F, Kull F, Ohlson E, Shafqat J, Thunnissen MMGM, Haeggström JZ: Leukotriene A4 hydrolase, insights into the molecular evolution by homology modeling and mutational analysis of enzyme from Saccharomyces cerevisiae. J Biol Chem. 2004, 280 (39): 33477–33486-Google Scholar
- Hori T, Yokomizo T, Ago H, Sugahara M, Ueno G, Yamamoto M, Kumasaka T, Shimizu T, Miyano M: Structural basis of leukotriene B-4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase catalytic mechanism and a possible Src homology 3 domain binding loop. Journal of Biological Chemistry. 2004, 279 (21): 22615-22623. 10.1074/jbc.M312655200.View ArticlePubMedGoogle Scholar
- Gillmor SA, Villaseñor A, Fletterick R, Sigal E, Browner MF: The structure of mammalian 15-lipoxygenase reveals similarity to the lipases and the determinants of substrate specificity. Nature Structural Biology. 1997, 4 (12): 1003-1009. 10.1038/nsb1297-1003.View ArticlePubMedGoogle Scholar
- Knight J, Taylor GW, Wright P, Clare AS, Rowley AF: Eicosanoid biosynthesis in an advanced deuterostomate invertebrate, the sea squirt (Ciona intestinalis). Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 1999, 1436 (3): 467-478. 10.1016/S0005-2760(98)00153-2.View ArticleGoogle Scholar
- Kuo JM, Pan BS, Zhang HJ, German JB: Identification of 12-Lipoxygenase in the hemolymph of tiger shrimp (Penaeus japonicus Bate). Journal of Agricultural and Food Chemistry. 1994, 42 (8): 1620-1623. 10.1021/jf00044a007.View ArticleGoogle Scholar
- Medeiros MN, Mendonca LH, Hunter AL, Paiva-Silva GO, Mello FG, Henze IP, Masuda H, Maya-Monteiro CM, Machado EA: The role of lipoxygenase products on the endocytosis of yolk proteins in insects: Participation of cAMP. Archives of Insect Biochemistry and Physiology. 2004, 55 (4): 178-187. 10.1002/arch.10129.View ArticlePubMedGoogle Scholar
- Sagi A, Silkovsky J, Fleisher-Berkovich S, Danon A, Chayoth R: Prostaglandin E(2) in previtellogenic ovaries of the prawn Macrobrachium rosenbergii: Synthesis and effect on the level of cAMP. General and Comparative Endocrinology. 1995, 100 (3): 308-313. 10.1006/gcen.1995.1161.View ArticlePubMedGoogle Scholar
- Silver RB, Oblak JB, Jeun GS, Sung JJ, Dutta TC: Leukotriene B4, an arachidonic acid metabolite, regulates intracellular free calcium release in eggs and mitotic cells of the sand dollar (Echinaracnius parma). Biological Bulletin. 1994, 187 (2): 242-244.PubMedGoogle Scholar
- Spaziani EP, Hinsch GW, Edwards SC: Changes in prostaglandin E(2) and F2-alpha during vitellogenesis in the Florida crayfish Procambarus paeninsulanus. Journal of Comparative Physiology B: Biochemical, Systemic and Environmental Physiology. 1993, 163 (7): 541-545. 10.1007/BF00302112.View ArticleGoogle Scholar
- Loher W, Ganjian I, Kubo I, Stanley-Samuelson D, Tobe SS: Prostaglandins - Their role in egg-laying of the cricket Teleogryllus commodus. Proceedings of the National Academy of Sciences of the United States of America. 1981, 78 (12): 7835-7838. 10.1073/pnas.78.12.7835.PubMed CentralView ArticlePubMedGoogle Scholar
- Miller JS, Nguyen T, Stanley-Samuelson DW: Eicosanoids mediate insect nodulation responses to bacterial infections. Proceedings of the National Academy of Sciences of the United States of America. 1994, 91 (26): 12418-12422. 10.1073/pnas.91.26.12418.PubMed CentralView ArticlePubMedGoogle Scholar
- KEGG: Kyoto Encyclopedia of Genes and Genomes. [http://www.genome.jp/kegg/]
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.