Dynamics of symbiont-mediated antibiotic production reveal efficient long-term protection for beewolf offspring
© Koehler et al.; licensee BioMed Central Ltd. 2013
Received: 27 September 2012
Accepted: 24 January 2013
Published: 31 January 2013
Insects have evolved a wide range of mechanisms to defend themselves and their offspring against antagonists. One of these strategies involves the utilization of antimicrobial compounds provided by symbiotic bacteria to protect the host or its nutritional resources from pathogens and parasites. In the symbiosis of the solitary digger wasp, Philanthus triangulum (Hymenoptera, Crabronidae), the bacterial symbiont ‘Candidatus Streptomyces philanthi’ defends the developing larvae against pathogens by producing a mixture of at least nine antimicrobial substances on the cocoon surface. This antibiotic cocktail inhibits the growth of a broad range of detrimental fungi and bacteria, thereby significantly enhancing the offspring’s survival probability.
Here we show that the production of antimicrobial compounds by the beewolf symbionts is confined to the first two weeks after cocoon spinning, leading to a high concentration of piericidins and streptochlorin on the cocoon surface. Expression profiling of housekeeping, sporulation, and antibiotic biosynthesis genes indicates that antibiotic production coincides with morphological differentiation that enables the symbionts to survive the nutrient-limited conditions on the beewolf cocoon. The antibiotic substances remain stable on the cocoon surface for the entire duration of the beewolf’s hibernation period, demonstrating that the compounds are resistant against environmental influences.
The antibiotic production by the beewolf symbionts serves as a reliable protection for the wasp offspring against pathogenic microorganisms during the long and unpredictable developmental phase in the subterranean brood cells. Thus, the beewolf-Streptomyces symbiosis provides one of the rare examples of antibiotics serving as an efficient defense in the natural environment and may aid in devising new strategies for the utilization of antibiotic combination therapies in human medicine against increasingly resistant bacterial and fungal pathogens.
KeywordsDefensive symbiosis Protective mutualism Crabronidae Antibiotic Streptomyces Hymenoptera Streptochlorin synthesis
Predators, parasites and pathogens can significantly reduce the reproductive success of insects. Developmental stages are especially prone to pathogen infestation given their often limited mobility as well as trade-offs that lead to limited resources being allocated into growth instead of defense[1–4]. Additionally, many insect species rear their offspring in subterranean nesting sites where the progeny is continuously threatened by soil pathogens[5–7]. To counteract these threats, insects have evolved an intricate immune system as well as chemical and behavioral defenses, including brood care, nesting in habitats which are difficult to invade for predators or brood parasites, and the application of antimicrobial substances as defense against pathogen infestation[7–10].
Sociality has been documented to provide insects with collective defensive behaviors aimed at eliminating parasites from group members and thereby limiting the spread of infections within the colony[11–14]. Specifically, allogrooming can serve as an efficient strategy to mechanically remove fungal spores, nematodes, mites and other parasites[11, 15, 16]. By contrast, solitary species are usually lacking these behavioral defenses[14, 17–19], making other means of protection of their offspring against fungal and bacterial pathogens particularly important. Although nest hygienic behaviors have also been observed in solitary insects, there is often no or only limited contact between adult and developing individuals, so chemical mechanisms are likely to play a more important role for antimicrobial defense[7, 20–22]. Concordantly, the production and use of various defensive compounds for the protection of the developing offspring has been described for a number of solitary insect species[8, 23–28].
In addition to the insect’s own defenses, recent studies have demonstrated that several taxa team up with bacterial symbionts to protect the host, the offspring or its nutritional resources against pathogens, predators, parasites, or parasitoids[20, 21, 29–33]. This protection can be mediated by (i) competitive exclusion of pathogenic microorganisms[34–36], (ii) interaction with the host’s immune system to enhance resistance against pathogenic infestation[17, 37, 38], or (iii) the production of chemicals that harm and/or deter antagonists[29, 33, 39–41].
Interestingly, many of the mutualistic microorganisms involved in defensive partnerships with insects belong to the bacterial phylum Actinobacteria[42, 43]. Members of this group appear to be predisposed towards engaging in defensive symbioses by their widespread distribution in the soil, the ability to subsist in nutritionally deficient environments, and, notably, their capacity to produce a wide variety of secondary metabolites with antimicrobial properties[40, 42, 44, 45]. These compounds may provide an efficient way to defend the producer against pathogenic or competing microorganisms, making Actinobacteria particularly suitable partners for defensive mutualistic interactions with insect hosts. Especially species of the genus Streptomyces are efficient producers of antimicrobial substances, with over two-thirds of the clinically relevant natural product antibiotics originating from this genus[44, 46]. Despite the common perception of antibiotics as agents of chemical warfare among competing microorganisms, evidence for the natural roles of these compounds and their defensive activity under in vivo conditions is still scarce[47, 48]. In fact, recent studies suggest that their primary effect in maintaining microbial communities may be the modulation of gene transcription at low concentrations rather than the elimination of competitors.
Fungus-farming ants represent a prime example of protective symbioses. The ants cultivate symbiotic fungi as a food source for their colony. To protect their source of nourishment from fungal infestation by the pathogenic fungus Escovopsis, the ants engage in a protective symbiosis with actinomycete bacteria which have been reported to produce antibiotic substances that inhibit the growth of Escovopsis[30, 41, 50–52], thereby protecting the fungal gardens without affecting the cultivar itself. Similarly, the fungus growing bark beetle Dendroctonus frontalis is associated with bacteria of the genus Streptomyces. The bacteria, present in the fungal galleries as well as in the mycangia of the beetle, produce the antifungal substance mycangimycin, which can protect the beetle’s nutritional resources against the antagonistic fungus Ophiostoma minus[33, 39].
Solitary digger wasps in the tribe Philanthini (‘beewolves’, Hymenoptera: Crabronidae) engage in a defensive symbiosis with bacteria of the genus Streptomyces for the protection of the developing offspring[20, 40, 54, 55]. Female beewolves cultivate the symbiotic microorganisms in specialized antennal gland reservoirs and secrete them into the brood cells prior to laying an egg on one of the provisioned bees. After oviposition, the female seals the brood cell with sand and subsequently does not provide any further brood care. As beewolf development can take up to nine months, including a long period of larval diapause during hibernation in the humid underground brood cells, the beewolf’s offspring is continuously threatened by pathogenic bacteria and fungi that may invade the brood cells from the surrounding soil as well as from the remains of the provisioned honeybee prey. As an efficient broad-spectrum defense, the larvae incorporate the symbiotic streptomycetes into the silken walls of their cocoons, where the symbionts produce at least nine different antibiotic substances that serve as an “antimicrobial combination prophylaxis” against pathogenic bacteria and fungi during the long and vulnerable period of hibernation.
The mutualistic association of beewolves and Streptomyces provides a unique opportunity to study the natural role of antimicrobial compounds in a symbiotic context[20, 40, 54, 58]. Here we investigated the dynamics of population size and antibiotic production of the symbiotic Streptomyces on the beewolf cocoon in order to elucidate the mechanisms that allow for the long-term defense against pathogens. The results yield insights into an efficient strategy for offspring protection in a solitary insect and provide a unique case study on the long-term efficacy of bacterial secondary metabolites directly in the natural environment.
Streptomyces population dynamics on beewolf cocoons
Dynamics of the antibiotic cocktail and piericidin gene expression
The discriminant analysis based on the relative amounts of streptochlorin (SC), piericidin A1 (PA1), and piericidin B1 (PB1) showed a significant change in the composition of the antibiotic cocktail over time, suggesting temporal differences in the production and/or deterioration of the single compounds (Wilks’ Lambda=0.432, Chi2=126.4, df=21, p<0.001; Figure 2b, Additional file1: Figure S1). In particular, we detected a decline in the relative amount of PA1 as compared to SC and PB1 towards the later time points, suggesting that production of PA1 starts earlier than that of SC and PB1, and that PA1 may be less stable than the other two compounds.
The expression of the putative piericidin gene (pks1) revealed a similar pattern as the chemical analyses, albeit shifted to earlier time points. Expression levels peaked at day 2 and 4 and subsequently decreased to background levels (Figure 2c, ANOVA: df=7, p<0.001).
Expression of housekeeping and sporulation genes
An improved understanding of the production and ecology of antimicrobial substances in natural environments is important to complement our present knowledge on the evolution of antibiotic resistance and to acquire new perspectives for the utilization of antibiotics in human medicine. The beewolf-Streptomyes symbiosis represents a unique model system to study these factors in vivo. Our results indicate that an “antibiotic cocktail” is produced by the defensive Streptomyces symbionts on beewolf cocoons within the first two weeks of larval development and subsequently serves as a reliable long-term antimicrobial protection for the wasp offspring. The stability and long-term efficacy of the symbiosis is mediated by morphological differentiation of the bacteria to survive unfavorable conditions on beewolf cocoons during larval hibernation and by the production of environmentally resistant antibiotics that remain stable on the cocoon as an efficient protection until emergence of the adult beewolf.
Growth and morphological differentiation of beewolf symbionts on the cocoon
Species of the genus Streptomyces are well known for the production of numerous small molecules that are biologically active outside of the cell. Many of those substances are inhibitors of enzymes and cellular processes that act as antimicrobials[48, 49, 59, 60], which help the producers to compete with antagonistic microorganisms or act as signaling molecules affecting gene transcription in co-occurring microbes[48, 49]. In many species, the production of antimicrobial secondary metabolites has been found to be tightly linked to morphological differentiation into inactive spores[59, 61–65]. In this context, the antimicrobial compounds may protect the cell’s metabolites from competitors during the vulnerable phase of re-allocation of the resources into spores. Prior studies of ‘Candidatus Streptomyces philanthi’ already proposed a morphological differentiation of the bacteria when applied to the surface of beewolf cocoons as a mechanism to survive the long period on the likely nutrient-limited cocoon surface during beewolf hibernation. Our results provide support for this hypothesis and indicate that the symbiont population only grows within the first two days following cocoon spinning and subsequently undergoes morphological differentiation (Figures 1 and3). Concordantly, two of the three analyzed housekeeping genes (ftsZ and EF-Tu) as well as the sporulation regulatory gene whiB show high expression levels during the first week on the cocoon, but then their expression is reduced to background levels (Figure 3a-c). WhiB, in particular, is known to be essential for early morphological differentiation as well as for the regulation of later sporulation gene transcription[66, 67]. It closely interacts with ftsZ, which regulates septum formation in normal cell division as well as during sporulation[68, 69]. Recent studies demonstrated that the expression of several regulatory whi genes, including whiB, is important for the correct timing of ftsZ and, as a result, for an optimal FtsZ protein level, which is essential for the formation of aerial biomass prior to morphological differentiation. The early onset of whiB and ftsZ expression on the cocoon (already at day 0) suggests that at least some of the symbiont cells already initiate morphological differentiation prior to incorporation into the cocoon silk. This is supported by the observation that low amounts of antibiotics can already be detected in the antennal gland secretion of female beewolves within the brood cell (T. Engl, S. Koehler, and M. Kaltenpoth, unpublished data).
Surprisingly, gyrB showed a much slower decrease in expression levels after day 8 as compared to the other genes (Figure 3d), suggesting that gyrase may play an important role during the later stages of morphological differentiation. Concordantly, previous studies in Bacillus subtilis and in E. coli[71–73] observed an inhibition of sporulation by gyrase inhibitors. Taken together, our gene expression analyses provide strong evidence for morphological differentiation of the beewolf symbionts into metabolically inactive spores about 1–2 weeks after cocoon spinning.
Dynamics of antibiotic production and PKS gene expression
As observed for free-living Streptomyces strains under in vitro conditions[63, 65, 74], morphological differentiation coincided with antibiotic production in ‘Ca. S. philanthi’. The expression profile of a gene within the putative piericidin biosynthesis gene cluster (pks1) [Nechitaylo et al., unpubl. data, and ref.] showed up-regulation between day 1 and day 4 after cocoon spinning, followed by down-regulation from day 8 onwards (Figure 2c). Accordingly, the highest amounts of antibiotics could be detected by GC-MS following the peak in antibiotic gene expression, i.e. between day 8 and 16 (Figure 2a). By producing antibiotic substances within the first two weeks after cocoon spinning, the symbionts establish a high concentration of antimicrobials on the surface of the cocoons. These compounds remain stable for months during hibernation, with an average total amount of 12.5 μg still present when the adult beewolf emerges. Even on nutrient-rich agar plates, this amount is high enough to effectively inhibit the growth of a wide range of soil fungi. Thus, the stability of the antibiotic substances allows them to serve as an efficient long-term defense throughout the beewolf’s developmental phase.
Stability of antibiotics mediates long-term antimicrobial protection for wasp offspring
In natural habitats, antibiotic compounds are subject to inactivation and degradation by different physicochemical and biological processes, e.g. photolysis, sorption, hydrolysis, and biodegradation[75–77]. Consequently, the stability varies greatly across compound classes and is strongly affected by abiotic and biotic conditions. While some substances are stable for months under aerobic conditions (e.g. fluorquinolones,[78, 79]), others (e.g. sulphonamides) experience rapid degradation and are cleared from the environment within 2–3 weeks[80, 81]. Polyene antibiotics, the class containing the piericidins, have been described as compounds which in solution are decomposed upon exposure to air, heat and light, while they show much higher stability (for up to several years) in dry state and in the absence of heat and light[83, 84]. In the beewolf symbiosis, the localization of the piericidins on the cocoon surface in the dark and moderate climate of the subterranean brood cell may explain how these compounds can stably subsist in the environment for a prolonged period of time. The long-term stability of the antibiotic cocktail is crucial for an efficient protection of the beewolf offspring during hibernation, and it is therefore strongly selected for on the holobiont level of the interacting partners.
In addition to the absolute amount of antibiotics, the composition of the cocktail changes on the cocoon surface over time (Figure 2b, Additional file1: Figure S1). PA1 is overrepresented in the first days after cocoon spinning, while its relative amount declines later on in favor of a higher proportion of PB1 and SC, suggesting differences in the timing of production as well as differential stability of the compounds. Possibly, PA1 is converted into PB1 by methylation of the hydroxyl group at the C16 position, which may explain the delayed increase and deterioration of PB1. The adaptive value of the changes in the antibiotic cocktail remains to be investigated. It is conceivable that the changing composition aids in preventing the emergence of resistant strains or serves to efficiently ward off a succession of different soil fungi and bacteria.
The importance of antibiotics for the wasp’s offspring
The stability of the symbiont-produced antimicrobial cocktail is essential for beewolves considering their brood care strategy. While social insects generally supply their progeny progressively with fresh food, mass provisioning is by far the most common strategy among solitary taxa[19, 87]. In mass provisioning species, the adult insect supplies all of the food for each offspring prior to laying the egg and subsequently closes the brood cell. This strategy has the advantage that a closed brood cell is less prone to invasion by parasitoids, e.g. cuckoo wasps. However, the risk of pathogen infestation in subterranean nesting sites is severe, especially in combination with the storage of resources. In progressive provisioners such as social insect societies, this problem is counteracted by worker insects continuously cleaning off pathogens and contaminated materials from developing brood and nestmates[11–13], or by applying antimicrobial compounds to limit the spread of pathogens. By contrast, mass-provisioning species like the European beewolf lack the possibility to continuously apply defensive chemicals or actively remove pathogens to protect their progeny. Therefore, the protection of the young with antimicrobial substances that are long lasting and resistant against environmental influences represents a particularly adaptive mechanism of parental care. In European beewolves, the symbiont-provided antibiotic treatment complements additional parental investments by the adult female to protect the offspring from pathogen infestation, such as embalming the prey bees with a postpharyngeal gland secretion to reduce fungal germination[5, 88, 89]. Altogether, these mechanisms ensure a successful completion of larval development for the beewolf progeny over their unpredictable developmental phase and serve as a reliable long term prophylaxis against pathogen attack.
It seems likely that long-term defensive strategies including symbiosis with antibiotic-producing microorganisms are more common among the large number of solitary insects that mass-provision their offspring in subterranean brood cells. However, the often unusual localization of protective symbionts as well as the inherent context-dependency of defensive symbioses hinder the detection of such associations. The increasing affordability of high-throughput sequencing technologies for microbial community analyses may at least partly ameliorate this problem by providing detailed insights into insect-associated microbial symbionts and uncovering candidates with putative protective functions.
In the beewolf-Streptomyces mutualism, symbiont-produced antibiotic substances have previously been found to significantly enhance the survival probability of the wasp’s offspring. Here we show that these compounds are produced only within a short period after larval cocoon spinning but subsequently remain stable on the cocoon surface for up to nine months of beewolf hibernation, thereby providing a reliable long-term protection for the developing beewolf larva against pathogens. Changes in the composition of the “antibiotic cocktail” over time indicate different production and degradation dynamics for the antibiotic compounds, which may aid to prevent the evolution of resistant microorganisms. The production of antibiotics on the cocoon coincides with morphological differentiation of the symbionts, which enables them to survive the inhospitable conditions during beewolf hibernation and to be successfully acquired from the cocoon surface by the next generation of emerging wasps. Thus, this symbiotic system appears to be finely tuned towards enhancing the efficiency of vertical symbiont transmission as well as providing long-term protection to the host offspring by warding off a broad range of pathogenic microorganisms. These findings provide new insights into the ecology of antibiotic production in the natural environment and may aid in exploring new strategies for the utilization of antibiotics in human medicine by using multi-drug combination therapies to counteract the increasing risk of resistant bacterial and fungal pathogens.
Beewolf rearing conditions
Female European beewolves, Philanthus triangulum, were obtained from a natural population in Berlin, Germany. The wasps were individually housed in observation cages situated in the greenhouse (14 h day, 10 h night; 23°C (+/− 3°C fluctuation) and provided ad libitum with honeybees (Apis mellifera) as prey and honey as food for the adult females within the cage.
Male beewolf cocoons were obtained from the females’ nests at different time points after cocoon spinning (day 0, 1, 2, 4, 8, and 16 after cocoon spinning). The cocoons were longitudinally cut to create an opening through which the larva was carefully removed using forceps. After dissection, the cocoon samples were directly stored at −80°C. Additionally, some cocoons with larvae were left to complete their development, and the cocoons were collected directly after the adult beewolf emerged from the cocoon. Some of these individuals developed directly within about four weeks (“emergence without hibernation”), while others went into diapause and emerged in the following spring (“emergence after hibernation”). All cocoon samples for emergence were transferred from the observation cages into open 1.5 ml Eppendorf cups approximately one week after cocoon spinning, placed in a box with moist sand and stored in an incubator at 26°C. Cocoons of individuals that developed directly and emerged within the same year were dissected immediately after emergence and frozen at −80°C. Individuals that had not entered the pupal stage four weeks after cocoon spinning were assumed to have entered diapause. These cocoons were transferred to the fridge and stored at 6°C for eight months over winter to mimic hibernating conditions. Subsequently, the samples were returned to the incubator at 26°C to induce pupation, so the cocoons in this group were about 9–10 months old upon sampling (eight months of diapause plus 4–8 weeks of development). After emergence of the adult beewolves, cocoons were stored at −80°C for subsequent analyses.
Frozen (−80°C) beewolf cocoons (n =157) were thawed on ice. The cocoons were transferred with forceps to GC-MS vials (1.5 ml vials, 6 mm jar opening, CZT, Kriftel, Germany), and 1 ml of methanol (>99.9%, Roth, Karlsruhe, Germany) was added to each sample. Forceps were cleaned in methanol and hexane in between samples to prevent contamination. Finally, the samples were placed on a shaker (Heidolph Vibramax 100) for 1 h at 350-400 rpm. After extraction, cocoons were taken out of the GC-MS vials, placed on a tissue to drain methanol and finally transferred to clean 1.5 ml cups (Eppendorf). Cocoon samples were placed under the hood for approximately 1 h to evaporate the remaining methanol for molecular analyses. Methanol extracts were stored at −20°C for subsequent GC-MS analysis.
GC-MS analysis of antibiotics
Methanol extracts were taken out of the freezer and evaporated to dryness under a gentle stream of Argon. Antibiotics were re-suspended in 50 μl of methanol and transferred to a 150 μl GC-μ-vial (CZT, Kriftel, Germany) for GC-MS analysis. An aliquot of 1 μl of each sample was injected into a Varian 450GC gas chromatograph coupled to a Varian 240MS mass spectrometer (Agilent Technologies, Böblingen, Germany) using a split/splitless injector at 250°C with the purge valve opened after 60 s. The GC was equipped with a DB5-MS capillary column (30 m × 0.25 mm diameter, film thickness: 0.25 μm, Agilent Technologies) and programmed from 150 to 300°C at 5°C/min with a 1min. initial isothermal and a 5min. final isothermal hold. Helium was used as carrier gas, with a constant flow rate of 1 ml/min. Mass spectra were recorded using electron ionization (EI-MS). Data acquisition and quantifications were achieved with MS Workstation Version 6.9.3 Software (Agilent Technologies). The five most abundant compounds on beewolf cocoons were quantified, i.e. streptochlorin (SC), piericidin A1 (PA1), piericidin B1 (PB1), piericidin A5 (PA5) and piericidin C1 (PC1). A dilution series (500–0.1ppm) of commercially available piericidin A1 was used as an external calibration standard for the four different piericidin derivatives, assuming similar ionization efficiencies based on the high structural similarity. For quantification of streptochlorin, we used a dilution series of a synthesized streptochlorin standard (for the synthesis of streptochlorin see Additional file2: Supplementary methods). The peaks were identified by comparison of their mass spectra with the standard spectra or with published reference spectra, and peak areas were automatically integrated using the MS Workstation Software. Finally, the success of this integration was controlled manually for every peak.
After methanol extraction, the dried beewolf cocoons were used for nucleic acid extraction. The cocoons were homogenized in liquid nitrogen followed by DNA/RNA extraction using the Epicentre MasterPure™ DNA extraction kit (Epicentre Technologies, Madison, USA). The kit is based on a precipitation reaction and can be used for combined DNA/RNA extraction. All solutions not included in the kit (TE buffer, water, 70% EtOH, Isopropanol) were prepared from RNase-free stock solutions. The protocol was adjusted as follows: the lysozyme treatment was omitted, and all centrifugation steps were done at room temperature. Finally, the DNA pellet was resuspended in 100 μl RNase-free low-TE buffer (1 mM Tris/HCl, 0.01 mM EDTA). Each sample was partitioned in two aliquots with 50 μl each and stored at −80°C.
Quantification of Streptomyces population size and gene expression
Primers used for PCR and quantitative PCR amplification of ‘ Ca . S. philanthi’ genes
Ampl icon length
Kaltenpoth et al., 2010
Kaltenpoth et al., 2010
Kaltenpoth et al., 2010
Kaltenpoth et al., 2010
To establish a standard curve for each gene of interest, DNA extracts of female beewolf antennae were thawed and used for PCR amplification. Amplification was performed on a VWR Gradient Thermocycler (UnoCycler, VWR, Darmstadt, Germany) in a total reaction volume of 12.5 μl, containing: 1 μl template DNA, 1×PCR buffer (key buffer, Tris/HCl, (NH4)2SO4 and 0.1% Tween 20), 2.5 mM MgCl2 (including the 1.5 mM MgCl2 in the buffer), 240 μM dNTPs, 0.8 μM of each primer and 0.5U Taq DNA-Polymerase (VWR, Darmstadt, Germany). Cycle parameters were as follows: an initial denaturation step at 94°C for 3 min, 35 cycles of 94°C for 40 s, 65°C for 40 s and 72°C for 40 s, and a final extension step of 72°C for 4 min. PCR success was verified by gel electrophoresis using a GelRed™ (Biotium, Hayward, USA) stained 1.5% TBE agarose gel (150V, 30 min). The documentation of gel pictures was conducted using GeneSnap image acquisition software (GeneSnap 7.09.06, Syngene, Camebridge, United Kingdom). Positive bands were excised from the gel and purified using PeqGold MicroSpin Gel extraction kit (Peqlab, Erlangen, Germany). The final DNA concentration of the purified PCR-product was obtained from NanoDrop measurements (ND1000 photo-spectrometer, PeqLab, Erlangen, Germany). Finally, the DNA samples were diluted to 1 ng/μl with RNase free water, and a serial dilution of 10-1 to 10-8 ng/μl was established to serve as a standard for qPCR quantification.
QPCR was used to quantify the Streptomyces population size on the surface of beewolf cocoons. Thawed DNA extracts of whole beewolf cocoons were used for qPCR amplification with ‘Ca. S. philanthi’ 16S rRNA gene-specific primers (Table 1) in a total reaction volume of 25 μl containing the following reagents: 6.5 μl RNase free water and 12.5 μl SYBR-Mix (Rotor-Gene SYBR Green RT-PCR kit, Qiagen, Hilden, Germany); 2.5 μl of each primer (10 μM), 1 μl template. Quantitative PCR was performed on a Rotor-Gene Q Cycler (Qiagen, Germany) using the following cycle parameters: 95°C for 10 min, 45 cycles of 95°C for 15 s, 60°C for 30 s, 72°C for 20 s, and a final melting curve analysis was performed by increasing the temperature from 72 to 95°C with 1°C gain. Based on the standard curve, the total amount of DNA was calculated based on the qPCR threshold values using the absolute quantification method[91, 92].
For quantification of the antibiotic gene expression as well as the expression of the housekeeping and sporulation genes, RNA extracts of whole beewolf cocoons were thawed and used for reverse transcription to obtain cDNA using the QuantiTect reverse transcription kit (Qiagen, Germany) and the specific primer pairs according to the manufacturer’s instructions. Subsequently, these cDNA samples were used for qPCR amplification with the same primers, using the same amplification conditions as indicated above.
Statistical analyses were performed using SPSS 17.0 Software (IBM, New York, USA). The amount of antibiotics estimated from GC-MS analysis as well as the symbiont 16S copy numbers and expression levels of housekeeping, antibiotic production, and sporulation genes estimated by qPCR were compared across different time points using ANOVA with Tukey posthoc measures. Additionally, changes in the composition of the antibiotic cocktail over time were analyzed based on the most abundant and consistently detected compounds, i.e. streptochlorin, piericidin A1 and piericidin B1. Omitting the compounds that were not consistently detected (PC1, PA5) is an approach that is conservative with regard to the hypothesis tested, i.e. that the composition of the cocktail changes over time. Single peak areas were translated into relative peak areas and subsequently log-ratio transformed using the Aitchison transformation. Finally, a discriminant analysis was used to elucidate differences in the composition of the antibiotic cocktail on beewolf cocoons over time (Figure 2b, Additional file1: Figure S1).
We thank Ulrike Helmhold and Benjamin Weiss for help with the beewolf rearing, and we gratefully acknowledge financial support from the Max Planck Society (SK, JD, and MK) and the German Science Foundation (MK, DFG KA2846/2-1).
- Rantala MJ, Roff DA: An analysis of trade-offs in immune function, body size and development time in the mediterranean field cricket, Gryllus bimaculatus. Funct Ecol. 2005, 19 (2): 323-330. 10.1111/j.1365-2435.2005.00979.x.View Article
- Vainio L, Hakkarainen H, Rantala MJ, Sorvari J: Individual variation in immune function in the ant Formica exsecta; effects of the nest, body size and sex. Evol Ecol. 2004, 18 (1): 75-84.View Article
- Soler JJ, de Neve L, Perez-Contreras T, Soler M, Sorci G: Trade-off between immunocompetence and growth in magpies: an experimental study. Proc Biol Sci. 2003, 270 (1512): 241-248. 10.1098/rspb.2002.2217.PubMed CentralView ArticlePubMed
- Schmid-Hempel P: Variation in immune defence as a question of evolutionary ecology. Proc Biol Sci. 2003, 270 (1513): 357-366. 10.1098/rspb.2002.2265.PubMed CentralView ArticlePubMed
- Herzner G, Strohm E: Fighting fungi with physics: food wrapping by a solitary wasp prevents water condensation. Curr Biol. 2007, 17 (2): R46-R47. 10.1016/j.cub.2006.11.060.View ArticlePubMed
- Janzen DH: Why fruits rot, seeds mold, and meat spoils. Am Nat. 1977, 111 (980): 691-713. 10.1086/283200.View Article
- Strohm E, Linsenmair KE: Females of the European beewolf preserve their honeybee prey against competing fungi. Ecol Entomol. 2001, 26 (2): 198-203. 10.1046/j.1365-2311.2001.00300.x.View Article
- Degenkolb T, During RA, Vilcinskas A: Secondary metabolites released by the burying beetle Nicrophorus vespilloides: chemical analyses and possible ecological functions. J Chem Ecol. 2011, 37 (7): 724-735. 10.1007/s10886-011-9978-4.View ArticlePubMed
- Bot ANM, Ortius-Lechner D, Finster K, Maile R, Boomsma JJ: Variable sensitivity of fungi and bacteria to compounds produced by the metapleural glands of leaf-cutting ants. Insectes Soc. 2002, 49 (4): 363-370. 10.1007/PL00012660.View Article
- Batra SWT: Behavior of some social and solitary halictine bees within their nests: a comparative study (Hymenoptera: Halicitidae). J Kans Entomol Soc. 1968, 41: 120-133.
- Reber A, Purcell J, Buechel SD, Buri P, Chapuisat M: The expression and impact of antifungal grooming in ants. J Evol Biol. 2011, 24 (5): 954-964. 10.1111/j.1420-9101.2011.02230.x.View ArticlePubMed
- Fefferman NH, Traniello JFA, Rosengaus RB, Calleri DV: Disease prevention and resistance in social insects: modeling the survival consequences of immunity, hygienic behavior, and colony organization. Behav Ecol Sociobiol. 2007, 61 (4): 565-577. 10.1007/s00265-006-0285-y.View Article
- Hughes WOH, Eilenberg J, Boomsma JJ: Trade-offs in group living: transmission and disease resistance in leaf-cutting ants. Proc Biol Sci. 2002, 269 (1502): 1811-1819. 10.1098/rspb.2002.2113.PubMed CentralView ArticlePubMed
- Cremer S, Armitage SAO, Schmid-Hempel P: Social immunity. Curr Biol. 2007, 17 (16): R693-R702. 10.1016/j.cub.2007.06.008.View ArticlePubMed
- Hamilton C, Lejeune BT, Rosengaus RB: Trophallaxis and prophylaxis: social immunity in the carpenter ant Camponotus pennsylvanicus. Biol Lett. 2011, 7 (1): 89-92. 10.1098/rsbl.2010.0466.PubMed CentralView ArticlePubMed
- Schmid-Hempel P: Parasites in social insects. 1998, Princeton: Princeton University Press
- Konrad M, Vyleta ML, Theis FJ, Stock M, Tragust S, Klatt M, Drescher V, Marr C, Ugelvig LV, Cremer S: Social transfer of pathogenic fungus promotes active immunisation in ant colonies. PLoS Biol. 2012, 10 (4): e1001300-10.1371/journal.pbio.1001300.PubMed CentralView ArticlePubMed
- Ugelvig LV, Cremer S: Social prophylaxis: group interaction promotes collective immunity in ant colonies. Curr Biol. 2007, 17 (22): 1967-1971. 10.1016/j.cub.2007.10.029.View ArticlePubMed
- Field J, Brace S: Pre-social benefits of extended parental care. Nature. 2004, 428 (6983): 650-652. 10.1038/nature02427.View ArticlePubMed
- Kaltenpoth M, Gottler W, Herzner G, Strohm E: Symbiotic bacteria protect wasp larvae from fungal infestation. Curr Biol. 2005, 15 (5): 475-479. 10.1016/j.cub.2004.12.084.View ArticlePubMed
- Kellner RLL: Molecular identification of an endosymbiotic bacterium associated with pederin biosynthesis in Paederus sabaeus (Coleoptera: Staphylinidae). Insect Biochem Mol Biol. 2002, 32 (4): 389-395. 10.1016/S0965-1748(01)00115-1.View ArticlePubMed
- Cane JH, Gerdin S, Wife G: Mandibular gland secretions of solitary bees (Hymenoptera, Apoidea) - potential for nest cell desinfection. J Kans Entomol Soc. 1983, 56 (2): 199-204.
- Rozen DE, Engelmoer DJP, Smiseth PT: Antimicrobial strategies in burying beetles breeding on carrion. Proc Natl Acad Sci USA. 2008, 105 (46): 17890-17895. 10.1073/pnas.0805403105.PubMed CentralView ArticlePubMed
- Marchini D, Marri L, Rosetto M, Manetti AGO, Dallai R: Presence of antibacterial peptides on the laid egg chorion of the medfly Ceratitis capitata. Biochem Biophys Res Commun. 1997, 240 (3): 657-663. 10.1006/bbrc.1997.7694.View ArticlePubMed
- Cotter SC, Kilner RM: Sexual division of antibacterial resource defence in breeding burying beetles, Nicrophorus vespilloides. J Anim Ecol. 2010, 79 (1): 35-43. 10.1111/j.1365-2656.2009.01593.x.View ArticlePubMed
- Burse A, Frick S, Discher S, Tolzin-Banasch K, Kirsch R, Strauss A, Kunert M, Boland W: Always being well prepared for defense: the production of deterrents by juvenile Chrysomelina beetles (Chrysomelidae). Phytochemistry. 2009, 70 (15–16): 1899-1909.View ArticlePubMed
- Discher S, Burse A, Tolzin-Banasch K, Heinemann SH, Pasteels JM, Boland W: A versatile transport network for sequestering and excreting plant glycosides in leaf beetles provides an evolutionary flexible defense strategy. Chem Bio Chem. 2009, 10 (13): 2223-2229. 10.1002/cbic.200900226.View ArticlePubMed
- Feld BK, Pasteels JM, Boland W: Phaedon cochleariae and Gastrophysa viridula (Coleoptera: Chrysomelidae) produce defensive iridoid monoterpenes de novo and are able to sequester glycosidically bound terpenoid precursors. Chemoecology. 2001, 11 (4): 191-198. 10.1007/PL00001851.View Article
- Mattoso TC, Moreira DD, Samuels RI: Symbiotic bacteria on the cuticle of the leaf-cutting ant Acromyrmex subterraneus subterraneus protect workers from attack by entomopathogenic fungi. Biol Lett. 2011, 8 (3): 461-464.PubMed CentralView ArticlePubMed
- Currie CR, Mueller UG, Malloch D: The agricultural pathology of ant fungus gardens. Proc Natl Acad Sci USA. 1999, 96 (14): 7998-8002. 10.1073/pnas.96.14.7998.PubMed CentralView ArticlePubMed
- Brownlie JC, Johnson KN: Symbiont-mediated protection in insect hosts. Trends Microbiol. 2009, 17 (8): 348-354. 10.1016/j.tim.2009.05.005.View ArticlePubMed
- Oliver KM, Russell JA, Moran NA, Hunter MS: Facultative bacterial symbionts in aphids confer resistance to parasitic wasps. Proc Natl Acad Sci USA. 2003, 100 (4): 1803-1807. 10.1073/pnas.0335320100.PubMed CentralView ArticlePubMed
- Scott JJ, Oh DC, Yuceer MC, Klepzig KD, Clardy J, Currie CR: Bacterial protection of beetle-fungus mutualism. Science. 2008, 322 (5898): 63-10.1126/science.1160423.PubMed CentralView ArticlePubMed
- Koch H, Schmid-Hempel P: Socially transmitted gut microbiota protect bumble bees against an intestinal parasite. Proc Natl Acad Sci USA. 2011, 108 (48): 19288-19292. 10.1073/pnas.1110474108.PubMed CentralView ArticlePubMed
- Dillon RJ, Dillon VM: The gut bacteria of insects: nonpathogenic interactions. Annu Rev Entomol. 2004, 49: 71-92. 10.1146/annurev.ento.49.061802.123416.View ArticlePubMed
- Forsgren E, Olofsson TC, Vásquez A, Fries I: Novel lactic acid bacteria inhibiting Paenibacillus larvae in honey bee larvae. Apidologie. 2009, 41 (1): 99-108.View Article
- Evans JD, Lopez DL: Bacterial probiotics induce an immune response in the honey bee (Hymenoptera: Apidae). J Econ Entomol. 2004, 97 (3): 752-756. 10.1603/0022-0493(2004)097[0752:BPIAIR]2.0.CO;2.View ArticlePubMed
- de Souza DJ, Bezier A, Depoix D, Drezen J-M, Lenoir A: Blochmannia endosymbionts improve colony growth and immune defence in the ant Camponotus fellah. BMC Microbiol. 2009, 9: 29-10.1186/1471-2180-9-29.PubMed CentralView ArticlePubMed
- Oh D-C, Scott JJ, Currie CR, Clardy J: Mycangimycin, a polyene peroxide from a mutualist Streptomyces sp. Org Lett. 2009, 11 (3): 633-636. 10.1021/ol802709x.PubMed CentralView ArticlePubMed
- Kroiss J, Kaltenpoth M, Schneider B, Schwinger MG, Hertweck C, Maddula RK, Strohm E, Svatos A: Symbiotic streptomycetes provide antibiotic combination prophylaxis for wasp offspring. Nat Chem Biol. 2010, 6 (4): 261-263. 10.1038/nchembio.331.View ArticlePubMed
- Currie CR, Scott JA, Summerbell RC, Malloch D: Fungus-growing ants use antibiotic-producing bacteria to control garden parasites. Nature. 1999, 398 (6729): 701-704. 10.1038/19519.View Article
- Kaltenpoth M: Actinobacteria as mutualists: general healthcare for insects?. Trends Microbiol. 2009, 17 (12): 529-535. 10.1016/j.tim.2009.09.006.View ArticlePubMed
- Seipke RF, Kaltenpoth M, Hutchings MI: Streptomyces as symbionts: an emerging and widespread theme?. FEMS Microbiol Rev. 2012, 36 (4): 862-876. 10.1111/j.1574-6976.2011.00313.x.View ArticlePubMed
- Watve MG, Tickoo R, Jog MM, Bhole BD: How many antibiotics are produced by the genus Streptomyces?. Arch Microbiol. 2001, 176 (5): 386-390. 10.1007/s002030100345.View ArticlePubMed
- Solecka J, Zajko J, Postek M, Rajnisz A: Biologically active secondary metabolites from actinomycetes. Cent Eur J Biol. 2012, 7 (3): 373-390. 10.2478/s11535-012-0036-1.
- Berdy J: Recent advances in and prospects of antibiotic research. Process Biochem. 1980, 15 (7): 28-35.
- Dufour N, Rao RP: Secondary metabolites and other small molecules as intercellular pathogenic signals. FEMS Microbiol Lett. 2011, 314 (1): 10-17. 10.1111/j.1574-6968.2010.02154.x.View ArticlePubMed
- Linares JF, Gustafsson I, Baquero F, Martinez JL: Antibiotics as intermicrobial signaling agents instead of weapons. Proc Natl Acad Sci USA. 2006, 103 (51): 19484-19489. 10.1073/pnas.0608949103.PubMed CentralView ArticlePubMed
- Yim G, Wang HH, Davies J: Antibiotics as signalling molecules. Philos T Roy Soc B. 2007, 362 (1483): 1195-1200. 10.1098/rstb.2007.2044.View Article
- Haeder S, Wirth R, Herz H, Spiteller D: Candicidin-producing Streptomyces support leaf-cutting ants to protect their fungus garden against the pathogenic fungus Escovopsis. Proc Natl Acad Sci USA. 2009, 106 (12): 4742-4746. 10.1073/pnas.0812082106.PubMed CentralView ArticlePubMed
- Cafaro MJ, Poulsen M, Little AE, Price SL, Gerardo NM, Wong B, Stuart AE, Larget B, Abbot P, Currie CR: Specificity in the symbiotic association between fungus-growing ants and protective Pseudonocardia bacteria. Proc Biol Sci. 2011, 278 (1713): 1814-1822. 10.1098/rspb.2010.2118.PubMed CentralView ArticlePubMed
- Poulsen M, Currie CR: Symbiont interactions in a tripartite mutualism: exploring the presence and impact of antagonism between two fungus-growing ant mutualists. PLoS One. 2010, 5 (1): e8748-10.1371/journal.pone.0008748.PubMed CentralView ArticlePubMed
- Poulsen M, Cafaro MJ, Erhardt DP, Little AEF, Gerardo NM, Tebbets B, Klein BS, Currie CR: Variation in Pseudonocardia antibiotic defence helps govern parasite-induced morbidity in Acromyrmex leaf-cutting ants. Environ Microbiol Rep. 2010, 2 (4): 534-540.PubMed CentralView ArticlePubMed
- Kaltenpoth M, Yildirim E, Guerbuez MF, Herzner G, Strohm E: Refining the roots of the beewolf-Streptomyces symbiosis: antennal symbionts in the rare genus Philanthinus (Hymenoptera, Crabronidae). Appl Environ Microbiol. 2012, 78 (3): 822-827. 10.1128/AEM.06809-11.PubMed CentralView ArticlePubMed
- Kaltenpoth M, Schmitt T, Polidori C, Koedam D, Strohm E: Symbiotic streptomycetes in antennal glands of the south American digger wasp genus Trachypus (Hymenoptera, Crabronidae). Physiol Entomol. 2010, 35 (2): 196-200. 10.1111/j.1365-3032.2010.00729.x.View Article
- Goettler W, Kaltenpoth M, Herzner G, Strohm E: Morphology and ultrastructure of a bacteria cultivation organ: the antennal glands of female european beewolves, Philanthus triangulum (hymenoptera, crabronidae). Arthropod Struct Dev. 2007, 36 (1): 1-9. 10.1016/j.asd.2006.08.003.View ArticlePubMed
- Strohm E, Linsenmair KE:Leaving the cradle: how beewolves (Philanthus triangulum F.) obtain the necessary spatial information for emergence. Zoology. 1995, 98: 137-146.
- Kaltenpoth M, Goettler W, Koehler S, Strohm E: Life cycle and population dynamics of a protective insect symbiont reveal severe bottlenecks during vertical transmission. Evol Ecol. 2010, 24 (2): 463-477. 10.1007/s10682-009-9319-z.View Article
- Chater KF, Biro S, Lee KJ, Palmer T, Schrempf H: The complex extracellular biology of Streptomyces. FEMS Microbiol Rev. 2010, 34 (2): 171-198. 10.1111/j.1574-6976.2009.00206.x.View ArticlePubMed
- Kitani S, Miyamoto KT, Takamatsu S, Herawati E, Iguchi H, Nishitomi K, Uchida M, Nagamitsu T, Omura S, Ikeda H: Avenolide, a Streptomyces hormone controlling antibiotic production in Streptomyces avermitilis. Proc Natl Acad Sci USA. 2011, 108 (39): 16410-16415. 10.1073/pnas.1113908108.PubMed CentralView ArticlePubMed
- Dharmaraj S: Marine Streptomyces as a novel source of bioactive substances. World J Microbiol Biotechnol. 2010, 26 (12): 2123-2139. 10.1007/s11274-010-0415-6.View Article
- Berdy J: New ways to obtain antibiotics. Chin J Antibiot. 1984, 7: 272-290.
- Champness WC, Chater KF: Regulation and integration of antibiotic production and morphological differentiation in Streptomyces spp. Regulation of bacterial differentiation. Edited by: Piggot P, Moran CP, Youngman P. 1994, Washington, DC: American Society for Microbiology, 61-93.
- Miyadoh S: Research on antibiotic screening in Japan over the last decade: a producing microorganisms approach. Actinomycetologica. 1993, 7: 100-106. 10.3209/saj.7_100.View Article
- Hopwood DA: Streptomyces in nature and medicine: the antibiotic makers. 2007, New York: Oxford University Press
- Chater KF: A morphological and genetic mapping study of white colony mutants of Streptomyces coelicolor. J Gen Microbiol. 1972, 72 (AUG): 9-28.View ArticlePubMed
- Ryding NJ, Bibb MJ, Molle V, Findlay KC, Chater KF, Buttner MJ: New sporulation loci in Streptomyces coelicolor A3(2). J Bacteriol. 1999, 181 (17): 5419-5425.PubMed CentralPubMed
- Willemse J, Mommaas AM, van Wezel GP: Constitutive expression of ftsZ overrides the whi developmental genes to initiate sporulation of Streptomyces coelicolor. Antonie van Leeuwenhoek. 2012, 101 (3): 619-632. 10.1007/s10482-011-9678-7.PubMed CentralView ArticlePubMed
- Flärdh K, Leibovitz E, Buttner MJ, Chater KF: Generation of a non-sporulating strain of Streptomyces coelicolor A3(2) by the manipulation of a developmentally controlled ftsZ promoter. Mol Microbiol. 2000, 38 (4): 737-749. 10.1046/j.1365-2958.2000.02177.x.View ArticlePubMed
- Vazquez-Ramos JM, Mandelstam J: Inhibition of sporulation by DNA gyrase inhibitors. J Gen Microbiol. 1981, 127: 11-17.PubMed
- Goss WA, Deitz WH, Cook TM: Mechanism of action of nalidixic acid on escherichia coli - inhibition of deoxyribonucleic acid synthesis. J Bacteriol. 1965, 89 (4): 1068-1074.PubMed CentralPubMed
- Staudenbauer WL: Replication of Escherichia coli DNA in vitro: inhibition by oxolinic acid. Eur J Biochem. 1976, 62 (3): 491-497. 10.1111/j.1432-1033.1976.tb10183.x.View ArticlePubMed
- Smith DH, Davis BD: Mode of action of novobiocin in Escherichia coli. J Bacteriol. 1967, 93 (1): 71-79.PubMed CentralPubMed
- van Wezel GP, McDowall KJ: The regulation of the secondary metabolism of Streptomyces: new links and experimental advances. Nat Prod Rep. 2011, 28 (7): 1311-1333. 10.1039/c1np00003a.View ArticlePubMed
- Huang CH, Renew JE, Pinkston K, Sedlak DL: Occurrence and fate of antibiotic compounds in municipal wastewater and animal waste. Proc Water Environ Fed. 2001, 686-697.
- Jefferys EG: The stability of antibiotics in soils. J Gen Microbiol. 1952, 7 (3–4): 295-312.View ArticlePubMed
- Kuehne M, Ihnen D, Moeller G, Agthe O: Stability of tetracycline in water and liquid manure. J Vet Med A. 2000, 47 (6): 379-384. 10.1046/j.1439-0442.2000.00300.x.View Article
- Golet EM, Strehler A, Alder AC, Giger W: Determination of fluoroquinolone antibacterial agents in sewage sludge and sludge-treated soil using accelerated solvent extraction followed by solid-phase extraction. Anal Chem. 2002, 74 (21): 5455-5462. 10.1021/ac025762m.View ArticlePubMed
- Giger W, Alder AC, Golet EM, Kohler HPE, McArdell CS, Molnar E, Siegrist H, Suter MJF: Occurrence and fate of antibiotics as trace contaminants in wastewaters, sewage sludges, and surface waters. Chimia. 2003, 57 (9): 485-491. 10.2533/000942903777679064.View Article
- Kreuzig R, Holtge S: Investigations on the fate of sulfadiazine in manured soil: laboratory experiments and test plot studies. Environ Toxicol Chem. 2005, 24 (4): 771-776. 10.1897/03-582R.1.View ArticlePubMed
- Schmidt B, Ebert J, Lamshoeft M, Thiede B, Schumacher-Buffel R, Ji R, Corvini PFX, Schaeffer A: Fate in soil of C-14-sulfadiazine residues contained in the manure of young pigs treated with a veterinary antibiotic. J Environ Sci Health B. 2008, 43 (1): 8-20. 10.1080/03601230701734824.View ArticlePubMed
- Oroshnik W, Mebane AD: The polyene antifungal antibiotics. Fort Chem Org Nat. 1963, 21: 17-79.
- Raab WP: Natamycin (pimaricin), its properties and possibilities in medicine. 1972, Stuttgart: Georg Thieme Verlag
- Bonner DP, Mechlinski W, Schaffner CP: Stability studies with amphotericin B and amphotericin B methyl ester. J Antibiot. 1975, 28: 132-135. 10.7164/antibiotics.28.132.View ArticlePubMed
- Mouton JW: Combination therapy as a tool to prevent emergence of bacterial resistance. Infection. 1999, 27: S24-S28.View ArticlePubMed
- Challis GL, Hopwood DA: Synergy and contingency as driving forces for the evolution of multiple secondary metabolite production by Streptomyces species. Proc Natl Acad Sci USA. 2003, 100: 14555-14561. 10.1073/pnas.1934677100.PubMed CentralView ArticlePubMed
- Field J: The evolution of progressive provisioning. Behav Ecol. 2005, 16 (4): 770-778. 10.1093/beheco/ari054.View Article
- Strohm E, Herzner G, Kaltenpoth M, Boland W, Schreier P, Geiselhardt S, Peschke K, Schmitt T: The chemistry of the postpharyngeal gland of female European beewolves. J Chem Ecol. 2008, 34 (5): 575-583. 10.1007/s10886-008-9447-x.PubMed CentralView ArticlePubMed
- Herzner G, Schmitt T, Peschke K, Hilpert A, Strohm E: Food wrapping with the postpharyngeal gland secretion by females of the European beewolf Philanthus triangulum. J Chem Ecol. 2007, 33 (4): 849-859. 10.1007/s10886-007-9263-8.View ArticlePubMed
- Liu Q, Yao F, Chooi YH, Kang Q, Xu W, Li Y, Shao Y, Shi Y, Deng Z, Tang Y: Elucidation of piericidin A1 biosynthetic locus revealed a thioesterase-dependent mechanism of alpha-pyridone ring formation. Chem Biol. 2012, 19 (2): 243-253. 10.1016/j.chembiol.2011.12.018.View ArticlePubMed
- Lee C, Lee SY, Shin SG, Hwang S: Real-time PCR determination of rRNA gene copy number: absolute and relative quantification assays with Escherichia coli. Appl Microbiol Biotechnol. 2008, 78 (2): 371-376. 10.1007/s00253-007-1300-6.View ArticlePubMed
- Lee C, Kim J, Shin SG, Hwang S: Absolute and relative qPCR quantification of plasmid copy number in Escherichia coli. J Biotechnol. 2006, 123 (3): 273-280. 10.1016/j.jbiotec.2005.11.014.View ArticlePubMed
- Aitchison J: The statistical analysis of compositional data. 1986, London: Chapman and HallView Article
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